7F5H

The crystal structure of RBD-Nanobody complex, DL28 (SC4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 65 2 2
Unit cell lengths	177.463, 177.463, 133.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.810 - 3.000
R-factor	0.22751
Rwork	0.226
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j 5m13
RMSD bond length	0.010
RMSD bond angle	1.582
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.174	1.565
Rmeas	0.184	1.652
Rpim	0.057	0.509
Number of reflections	25343	2472
<I/σ(I)>	14.1	1.35
Completeness [%]	100.0	100
Redundancy	9.2	9.7
CC(1/2)	0.990	0.569

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M Potassium phosphate dibasic, 20% PEG3350