7F24

Cryo-EM structure of the GTP-bound dopamine receptor 1 and mini-Gs complex without Nb35

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	248	28907.7	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-stimulating G alpha protein
2	B (F)	D(1A) dopamine receptor	polymer	473	52409.7	1	UniProt (P21728) Pfam (PF00001)	Homo sapiens (Human)	Dopamine D1 receptor
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (Human)	Transducin beta chain 1
4	D (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
5	E (A)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466)
6	F (F)	L-DOPAMINE	non-polymer		153.2	1	Chemie (LDP) PubChem (681)

Sequence modifications

A: 6 - 64 (UniProt: P63092)

PDB	External Database	Details
Asp 49	Gly 49	engineered mutation
Asn 50	Glu 50	engineered mutation

A: 204 - 394 (UniProt: P63092)

PDB	External Database	Details
Gly 65	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 235	engineered mutation
Asp 252	Ser 238	engineered mutation
-	Met 241	deletion
-	Val 242	deletion
-	Ile 243	deletion
-	Arg 244	deletion
-	Glu 245	deletion
-	Asp 246	deletion
-	Asn 247	deletion
-	Gln 248	deletion
-	Thr 249	deletion
-	Asn 250	deletion
Ala 372	Ile 358	engineered mutation
Ile 375	Val 361	engineered mutation

F: 1 - 446 (UniProt: P21728)

PDB	External Database	Details
Met -26	-	initiating methionine
Lys -25	-	expression tag
Thr -24	-	expression tag
Ile -23	-	expression tag
Ile -22	-	expression tag
Ala -21	-	expression tag
Leu -20	-	expression tag
Ser -19	-	expression tag
Tyr -18	-	expression tag
Ile -17	-	expression tag
Phe -16	-	expression tag
Cys -15	-	expression tag
Leu -14	-	expression tag
Val -13	-	expression tag
Phe -12	-	expression tag
Ala -11	-	expression tag
Asp -10	-	expression tag
Tyr -9	-	expression tag
Lys -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Ala -3	-	expression tag
Ser -2	-	expression tag
Ile -1	-	expression tag
Asp 0	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

D: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	128580.5
Non-Polymers*	Number of molecules	2
	Total formula weight	676.4
All*	Total formula weight	129256.9

*Water molecules are not included.