7F24
Cryo-EM structure of the GTP-bound dopamine receptor 1 and mini-Gs complex without Nb35
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.16 |
| Number of Particle | 1968728 |
| Software | cryoSPARC,RELION |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
