7ET1
Cryo-EM structure of the gastric proton pump K791S/E820D/Y340N/E936V/Y799W mutant in K+-occluded (K+)E2-AlF state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Sodium/potassium-transporting ATPase subunit alpha | polymer | 987 | 109099.5 | 1 | UniProt (A0A5G2QYH2) Pfam (PF00690) Pfam (PF00122) Pfam (PF13246) Pfam (PF08282) Pfam (PF00689) UniProt (by SIFTS) (P19156) | Sus scrofa (Pig) | |
| 2 | B (B) | Potassium-transporting ATPase subunit beta | polymer | 262 | 29762.1 | 1 | UniProt (P18434) Pfam (PF00287) | Sus scrofa (Pig) | Gastric H(+)/K(+) ATPase subunit beta,Proton pump beta chain,gp60-90 |
| 3 | C (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 4 | D, E, K (A, B) | 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 787.1 | 3 | Chemie (PCW) | |||
| 5 | F, I, J (A) | POTASSIUM ION | non-polymer | 39.1 | 3 | Chemie (K) PubChem (813) | |||
| 6 | G (A) | TETRAFLUOROALUMINATE ION | non-polymer | 103.0 | 1 | Chemie (ALF) PubChem (5311325) | |||
| 7 | H (A) | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) PubChem (16219173) PubChem (16758052) PubChem (304) PubChem (176980071) PubChem (40477506) PubChem (40557614) PubChem (42579431) PubChem (42579432) PubChem (18640088) PubChem (1715243) PubChem (1715244) PubChem (45044259) PubChem (23423981) PubChem (49853509) PubChem (50990013) PubChem (52934537) PubChem (56621683) PubChem (191463) PubChem (57915018) PubChem (57939063) PubChem (58428240) PubChem (58445498) PubChem (58663286) PubChem (368063) PubChem (369319) PubChem (44654663) PubChem (59450329) PubChem (59628412) PubChem (59965215) PubChem (71234641) PubChem (59993755) PubChem (60001463) PubChem (66634651) PubChem (68055877) PubChem (68166463) PubChem (68315613) PubChem (46937002) PubChem (70795838) PubChem (70797612) PubChem (70954610) PubChem (70957185) PubChem (71463909) PubChem (638019) PubChem (59274407) PubChem (1549081) PubChem (1553803) PubChem (68311425) PubChem (68311398) PubChem (143173313) PubChem (177156123) PubChem (89951918) PubChem (90224410) PubChem (5997) PubChem (5283629) PubChem (5284644) PubChem (1551269) PubChem (90070115) PubChem (91298236) PubChem (5315842) PubChem (117965027) PubChem (6432564) PubChem (6710666) PubChem (124146240) PubChem (7059399) PubChem (124897607) PubChem (124896959) PubChem (6419195) PubChem (10861996) PubChem (132837164) PubChem (101624109) PubChem (6994560) PubChem (7055705) PubChem (7055706) PubChem (7059398) PubChem (7059400) PubChem (7059635) PubChem (7103033) PubChem (7182477) PubChem (7182478) PubChem (11834483) PubChem (11888915) PubChem (11888914) PubChem (9909206) PubChem (10045721) PubChem (71168115) PubChem (10134774) PubChem (10293553) PubChem (71173031) PubChem (124225449) PubChem (138967089) PubChem (11003580) PubChem (11025495) PubChem (11058155) PubChem (139566851) PubChem (124429284) PubChem (11463329) PubChem (11868624) PubChem (11868625) PubChem (11869366) PubChem (11869367) PubChem (11869368) PubChem (11869369) PubChem (12120344) PubChem (12156156) PubChem (12303135) PubChem (12303148) PubChem (12303156) PubChem (12303159) PubChem (12303160) PubChem (15939944) PubChem (124909223) PubChem (124909224) PubChem (124909225) PubChem (171828367) PubChem (173516975) PubChem (130459183) PubChem (131992107) PubChem (139568882) PubChem (142983773) PubChem (144054483) PubChem (144209461) PubChem (144331236) PubChem (144808354) PubChem (145053568) PubChem (145157652) PubChem (145215073) PubChem (145230478) PubChem (146320991) PubChem (147857230) PubChem (154993519) PubChem (158603858) PubChem (162906452) PubChem (162906453) PubChem (162906454) PubChem (162906455) PubChem (162906456) PubChem (162906457) PubChem (163255768) PubChem (163389210) PubChem (164665303) PubChem (166553771) PubChem (166712004) PubChem (170116175) PubChem (171764280) PubChem (171817101) PubChem (171817104) PubChem (174285573) | |||
| 8 | L, M, N, O, P (B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 5 | Chemie (NAG) | |||
| 9 | Q (A) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A: 48 - 1033 (UniProt: A0A5G2QYH2)
| PDB | External Database | Details |
|---|---|---|
| Ala 47 | - | expression tag |
| Asn 340 | Tyr 348 | engineered mutation |
| Ser 791 | Lys 799 | engineered mutation |
| Trp 799 | Tyr 807 | engineered mutation |
| Asp 820 | Glu 828 | engineered mutation |
| Val 936 | Glu 944 | engineered mutation |
| Ser 1005 | Gly 1013 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 138861.7 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 4098.6 | |
| All* | Total formula weight | 142960.3 |
