7ET1
Cryo-EM structure of the gastric proton pump K791S/E820D/Y340N/E936V/Y799W mutant in K+-occluded (K+)E2-AlF state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Sodium/potassium-transporting ATPase subunit alpha | polymer | 987 | 109099.5 | 1 | UniProt (A0A5G2QYH2) Pfam (PF00690) Pfam (PF00122) Pfam (PF13246) Pfam (PF00702) Pfam (PF00689) UniProt (by SIFTS) (P19156) | Sus scrofa (Pig) | |
| 2 | B (B) | Potassium-transporting ATPase subunit beta | polymer | 262 | 29762.1 | 1 | UniProt (P18434) Pfam (PF00287) | Sus scrofa (Pig) | Gastric H(+)/K(+) ATPase subunit beta,Proton pump beta chain,gp60-90 |
| 3 | C (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 4 | D, E, K (A, B) | 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 787.1 | 3 | Chemie (PCW) | |||
| 5 | F, I, J (A) | POTASSIUM ION | non-polymer | 39.1 | 3 | Chemie (K) | |||
| 6 | G (A) | TETRAFLUOROALUMINATE ION | non-polymer | 103.0 | 1 | Chemie (ALF) | |||
| 7 | H (A) | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) | |||
| 8 | L, M, N, O, P (B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 5 | Chemie (NAG) | |||
| 9 | Q (A) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A: 48 - 1033 (UniProt: A0A5G2QYH2)
| PDB | External Database | Details |
|---|---|---|
| Ala 47 | - | expression tag |
| Asn 340 | Tyr 348 | engineered mutation |
| Ser 791 | Lys 799 | engineered mutation |
| Trp 799 | Tyr 807 | engineered mutation |
| Asp 820 | Glu 828 | engineered mutation |
| Val 936 | Glu 944 | engineered mutation |
| Ser 1005 | Gly 1013 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 138861.7 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 4098.6 | |
| All* | Total formula weight | 142960.3 |
