7EGQ
Co-transcriptional capping machineries in SARS-CoV-2 RTC: Coupling of N7-methyltransferase and 3'-5' exoribonuclease with polymerase reveals mechanisms for capping and proofreading
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, I (A, N) | RNA-directed RNA polymerase | polymer | 932 | 106781.0 | 2 | UniProt (P0DTD1) Pfam (PF06478) Pfam (PF00680) | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) | Pol, RdRp, Non-structural protein 12, nsp12 |
| 2 | B, D, J, L (B, D, O, Q) | Non-structural protein 8 | polymer | 198 | 21903.0 | 4 | UniProt (P0DTD1) Pfam (PF08717) | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) | nsp8 |
| 3 | C, K (C, P) | Non-structural protein 7 | polymer | 83 | 9248.8 | 2 | UniProt (P0DTD1) Pfam (PF08716) | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2) | nsp7 |
| 4 | E, M, Q, R (E, R, F, S) | Helicase | polymer | 601 | 66930.5 | 4 | UniProt (P0DTD1) Pfam (PF20632) Pfam (PF20633) Pfam (PF20631) Pfam (PF13087) | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) | Hel, Non-structural protein 13, nsp13 |
| 5 | F, N (G, T) | Non-structural protein 9 | polymer | 117 | 12794.6 | 2 | UniProt (P0DTD1) Pfam (PF08710) | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) | nsp9 |
| 6 | G, O (H, U) | Non-structural protein 10 | polymer | 139 | 14802.9 | 2 | UniProt (P0DTD1) Pfam (PF09401) | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2) | nsp10,Growth factor-like peptide,GFL |
| 7 | H, P (K, X) | Proofreading exoribonuclease | polymer | 527 | 59887.5 | 2 | UniProt (P0DTD1) Pfam (PF06471) | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2) | ExoN,Guanine-N7 methyltransferase,Non-structural protein 14,nsp14 |
| 8 | S, U (I, L) | primer RNA | polymer | 25 | 8172.9 | 2 | Severe acute respiratory syndrome coronavirus 2 | ||
| 9 | T, V (J, M) | Template RNA | polymer | 33 | 10443.2 | 2 | Severe acute respiratory syndrome coronavirus 2 | ||
| 10 | AA, BA, CA, DA, EA... (E, H, K, N, R...) | ZINC ION | non-polymer | 65.4 | 26 | Chemie (ZN) | |||
| 11 | GA, HA, SA (K, X) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) |
Sequence modifications
G, T: 1 - 113 (UniProt: P0DTD1)
| PDB | External Database | Details |
|---|---|---|
| Ser -3 | - | expression tag |
| Asn -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Met 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 22 |
| Total formula weight | 799596.2 | |
| Non-Polymers* | Number of molecules | 29 |
| Total formula weight | 1773.5 | |
| All* | Total formula weight | 801369.8 |
