7EA9

Crystal Structure of human lysyl-tRNA synthetase Y145H mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Lysine--tRNA ligase	polymer	521	60286.3	4	UniProt (Q15046) Pfam (PF01336) Pfam (PF00152) In PDB	Homo sapiens (Human)	Lysyl-tRNA synthetase,LysRS
2	A, B, C, D	5'-O-[(L-LYSYLAMINO)SULFONYL]ADENOSINE	non-polymer		474.5	4	Chemie (KAA)
3	A, B, C, D	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4		water	water		18.0	546	Chemie (HOH)

Sequence modifications

A, B, C, D: 70 - 581 (UniProt: Q15046)

PDB	External Database	Details
Met 69	-	initiating methionine
His 145	Tyr 145	engineered mutation
Leu 582	-	expression tag
Glu 583	-	expression tag
His 584	-	expression tag
His 585	-	expression tag
His 586	-	expression tag
His 587	-	expression tag
His 588	-	expression tag
His 589	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	241145.1
Non-Polymers*	Number of molecules	8
	Total formula weight	2266.3
All*	Total formula weight	243411.5

*Water molecules are not included.