7E9G

Cryo-EM structure of Gi-bound metabotropic glutamate receptor mGlu2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (R, S)	Metabotropic glutamate receptor 2	polymer	817	90240.3	2	UniProt (Q14416) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003)	Homo sapiens (Human)	mGluR2
2	C (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
3	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
4	E (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
5	F (D)	scFv16	polymer	257	27409.6	1		Homo sapiens
6	G, H (E, F)	DN13	polymer	124	13637.2	2		Lama glama
7	I, K (R, S)	(1S,2S,5R,6S)-2-aminobicyclo[3.1.0]hexane-2,6-dicarboxylic acid	non-polymer		185.2	2	Chemie (40F)
8	J (R)	1-butyl-3-chloranyl-4-(4-phenylpiperidin-1-yl)pyridin-2-one	non-polymer		344.9	1	Chemie (HZR)

Sequence modifications

R, S: 19 - 825 (UniProt: Q14416)

PDB	External Database	Details
Asp 9	-	expression tag
Tyr 10	-	expression tag
Lys 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Asp 15	-	expression tag
Gly 16	-	expression tag
Ala 17	-	expression tag
Pro 18	-	expression tag
Ala 601	Ser 601	conflict

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	322184.0
Non-Polymers*	Number of molecules	3
	Total formula weight	715.2
All*	Total formula weight	322899.3

*Water molecules are not included.