7E9G
Cryo-EM structure of Gi-bound metabotropic glutamate receptor mGlu2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (R, S) | Metabotropic glutamate receptor 2 | polymer | 817 | 90240.3 | 2 | UniProt (Q14416) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003) | Homo sapiens (Human) | mGluR2 |
| 2 | C (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | D (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 351 | 38744.4 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (Human) | Transducin beta chain 1 |
| 4 | E (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 5 | F (D) | scFv16 | polymer | 257 | 27409.6 | 1 | Homo sapiens | ||
| 6 | G, H (E, F) | DN13 | polymer | 124 | 13637.2 | 2 | Lama glama | ||
| 7 | I, K (R, S) | (1S,2S,5R,6S)-2-aminobicyclo[3.1.0]hexane-2,6-dicarboxylic acid | non-polymer | 185.2 | 2 | Chemie (40F) PubChem (114827) PubChem (213056) PubChem (6101858) PubChem (10702665) PubChem (10845212) PubChem (44322702) PubChem (45098631) PubChem (50989828) PubChem (156666) PubChem (15408708) PubChem (89156372) PubChem (89651893) PubChem (90488818) PubChem (90936348) PubChem (90981390) PubChem (92403463) PubChem (98137211) PubChem (99722638) PubChem (99722640) PubChem (101932822) PubChem (102066259) PubChem (121226785) PubChem (124560124) PubChem (130820172) PubChem (130976542) PubChem (131013715) PubChem (139025891) PubChem (146157633) PubChem (154825295) | |||
| 8 | J (R) | 1-butyl-3-chloranyl-4-(4-phenylpiperidin-1-yl)pyridin-2-one | non-polymer | 344.9 | 1 | Chemie (HZR) PubChem (25195461) |
Sequence modifications
R, S: 19 - 825 (UniProt: Q14416)
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asp 9 | - | expression tag |
| Tyr 10 | - | expression tag |
| Lys 11 | - | expression tag |
| Asp 12 | - | expression tag |
| Asp 13 | - | expression tag |
| Asp 14 | - | expression tag |
| Asp 15 | - | expression tag |
| Gly 16 | - | expression tag |
| Ala 17 | - | expression tag |
| Pro 18 | - | expression tag |
| Ala 601 | Ser 601 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 322184.0 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 715.2 | |
| All* | Total formula weight | 322899.3 |
