7E7F

Human CYP11B1 mutant in complex with metyrapone

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cytochrome P450 11B1, mitochondrial	polymer	486	55821.3	1	UniProt (P15538) Pfam (PF00067) In PDB	Homo sapiens (Human)	CYPXIB1,Cytochrome P-450c11,Cytochrome P450C11,Steroid 11-beta-hydroxylase,CYP11B1
2	A	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	1	Chemie (HEM)
3	A	METYRAPONE	non-polymer		226.3	1	Chemie (MYT)
4	A	CHOLIC ACID	non-polymer		408.6	2	Chemie (CHD)
5	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6		water	water		18.0	418	Chemie (HOH)

Sequence modifications

A: 28 - 503 (UniProt: P15538)

PDB	External Database	Details
Met 24	-	initiating methionine
Ala 25	-	expression tag
Thr 26	-	expression tag
Lys 27	-	expression tag
Arg 49	Trp 49	engineered mutation
Asn 50	Leu 50	engineered mutation
Asn 53	Leu 53	engineered mutation
Arg 56	Trp 56	engineered mutation
Asn 244	Leu 244	engineered mutation
Asn 247	Trp 247	engineered mutation
His 504	-	expression tag
His 505	-	expression tag
His 506	-	expression tag
His 507	-	expression tag
His 508	-	expression tag
His 509	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	55821.3
Non-Polymers*	Number of molecules	6
	Total formula weight	1844.1
All*	Total formula weight	57665.4

*Water molecules are not included.