7E2H

Cryo-EM structure of hDisp1NNN-3C-Cleavage

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	D	Protein dispatched homolog 1	polymer	270	29497.6	1	UniProt (Q96F81) In PDB	Homo sapiens (Human)
2	E	Protein dispatched homolog 1	polymer	1248	140427.6	1	UniProt (Q96F81) Pfam (PF02460) In PDB	Homo sapiens (Human)
3	E	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	8	Chemie (Y01)
4	E	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)

Sequence modifications

D: 1 - 262 (UniProt: Q96F81)

PDB	External Database	Details
Ser 263	-	linker
Ser 264	-	linker
Leu 271	-	linker
Glu 272	-	linker
Val 273	-	linker
Leu 274	-	linker
Phe 275	-	linker
Gln 276	-	linker

E: 281 - 1524 (UniProt: Q96F81)

PDB	External Database	Details
Gly 277	-	linker
Pro 278	-	linker
Gly 279	-	linker
Ser 280	-	linker
Asn 572	Asp 572	engineered mutation
Asn 573	Asp 573	engineered mutation
Asn 1051	Asp 1051	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	169925.2
Non-Polymers*	Number of molecules	12
	Total formula weight	4778.6
All*	Total formula weight	174703.9

*Water molecules are not included.