7DCO
Cryo-EM structure of the activated spliceosome (Bact complex) at an atomic resolution of 2.5 angstrom
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | PRP8 isoform 1 | polymer | 2413 | 279867.5 | 1 | UniProt (A0A6A5PW68) | Saccharomyces cerevisiae (Baker's yeast) | |
| 2 | B (B) | U5 snRNA | polymer | 214 | 68643.3 | 1 | GenBank (1039023403) | Saccharomyces cerevisiae | |
| 3 | C (C) | SNU114 isoform 1 | polymer | 1008 | 114174.0 | 1 | UniProt (A0A6A5PW35) | Saccharomyces cerevisiae (Baker's yeast) | |
| 4 | D (D) | BRR2 isoform 1 | polymer | 2163 | 246470.3 | 1 | UniProt (A0A6A5Q2I8) | Saccharomyces cerevisiae (Baker's yeast) | |
| 5 | E (d) | Small nuclear ribonucleoprotein Sm D3 | polymer | 101 | 11240.1 | 1 | UniProt (A0A6A5Q3H8) | Saccharomyces cerevisiae (Baker's yeast) | Sm-D3,snRNP core protein D3 |
| 6 | F, Q (a, h) | BJ4_G0014900.mRNA.1.CDS.1 | polymer | 196 | 22427.0 | 2 | UniProt (A0A6A5Q0I3) | Saccharomyces cerevisiae (Baker's yeast) | HLJ1_G0014660.mRNA.1.CDS.1,SMB1 isoform 1,Y55_G0014800.mRNA.1.CDS.1 |
| 7 | G, V (b, m) | SMD1 isoform 1 | polymer | 146 | 16296.8 | 2 | UniProt (A0A6A5Q0W7) | Saccharomyces cerevisiae (Baker's yeast) | |
| 8 | H, W (c, n) | BJ4_G0037700.mRNA.1.CDS.1 | polymer | 110 | 12876.1 | 2 | UniProt (A0A6L0ZK95) | Saccharomyces cerevisiae (Baker's yeast) | HLJ1_G0037800.mRNA.1.CDS.1,HN1_G0037500.mRNA.1.CDS.1,Y55_G0037640.mRNA.1.CDS.1 |
| 9 | I, R (e, i) | Small nuclear ribonucleoprotein E | polymer | 94 | 10385.1 | 2 | UniProt (A0A6A5Q895) | Saccharomyces cerevisiae (Baker's yeast) | snRNP-E,Sm protein E |
| 10 | J, S (f, j) | Sm protein F | polymer | 86 | 9669.9 | 2 | UniProt (A0A6A5Q5K4) | Saccharomyces cerevisiae (Baker's yeast) | |
| 11 | K, T (g, k) | Small nuclear ribonucleoprotein G | polymer | 77 | 8490.8 | 2 | UniProt (P40204) Pfam (PF01423) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | snRNP-G,Sm protein G,SmG |
| 12 | L (F) | U6 snRNA | polymer | 112 | 35883.2 | 1 | GenBank (1039022925) | Saccharomyces cerevisiae | |
| 13 | M (G) | pre-mRNA | polymer | 162 | 44772.2 | 1 | Saccharomyces cerevisiae | ||
| 14 | N (H) | U2 snRNA | polymer | 1175 | 376324.6 | 1 | Saccharomyces cerevisiae | ||
| 15 | O (o) | HLJ1_G0053790.mRNA.1.CDS.1 | polymer | 238 | 27232.3 | 1 | UniProt (A0A6A5Q2G3) | Saccharomyces cerevisiae (Baker's yeast) | LEA1 isoform 1,Y55_G0053650.mRNA.1.CDS.1 |
| 16 | P (p) | BJ4_G0027490.mRNA.1.CDS.1 | polymer | 111 | 12850.9 | 1 | UniProt (A0A6A5Q318) | Saccharomyces cerevisiae (Baker's yeast) | HN1_G0027200.mRNA.1.CDS.1,MSL1 isoform 1,SX2_G0029270.mRNA.1.CDS.1,Y55_G0027550.mRNA.1.CDS.1 |
| 17 | U (l) | Small nuclear ribonucleoprotein Sm D3 | polymer | 81 | 8882.2 | 1 | UniProt (A0A6A5Q3H8) | Saccharomyces cerevisiae (Baker's yeast) | Sm-D3,snRNP core protein D3 |
| 18 | X (u) | PRP9 isoform 1 | polymer | 530 | 63126.4 | 1 | UniProt (A0A6A5PZ67) | Saccharomyces cerevisiae (Baker's yeast) | |
| 19 | Y (w) | Pre-mRNA-splicing factor PRP21 | polymer | 280 | 33111.5 | 1 | UniProt (P32524) Pfam (PF12230) Pfam (PF01805) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 20 | Z (v) | PRP11 isoform 1 | polymer | 266 | 29962.8 | 1 | UniProt (A0A6A5Q100) | Saccharomyces cerevisiae (Baker's yeast) | |
| 21 | AA (1) | HSH155 isoform 1 | polymer | 971 | 110166.7 | 1 | UniProt (A0A6A5PSM1) | Saccharomyces cerevisiae (Baker's yeast) | |
| 22 | BA (2) | HLJ1_G0043010.mRNA.1.CDS.1 | polymer | 238 | 27503.8 | 1 | UniProt (A0A6L0ZSI9) | Saccharomyces cerevisiae (Baker's yeast) | |
| 23 | CA (3) | RSE1 isoform 1 | polymer | 1361 | 153956.8 | 1 | UniProt (A0A6A5PUP5) | Saccharomyces cerevisiae (Baker's yeast) | |
| 24 | DA (4) | HSH49 isoform 1 | polymer | 213 | 24534.2 | 1 | UniProt (A0A6A5Q466) | Saccharomyces cerevisiae (Baker's yeast) | |
| 25 | EA (5) | BJ4_G0056610.mRNA.1.CDS.1 | polymer | 107 | 12283.6 | 1 | UniProt (A0A6A5Q1K7) | Saccharomyces cerevisiae (Baker's yeast) | EM14S01-3B_G0056430.mRNA.1.CDS.1,HLJ1_G0056610.mRNA.1.CDS.1,HN1_G0056420.mRNA.1.CDS.1,JXXY16.1_G0056310.mRNA.1.CDS.1,RDS3 isoform 1,SX2_G0056170.mRNA.1.CDS.1,XXYS1_G0055940.mRNA.1.CDS.1,Y55_G0056510.mRNA.1.CDS.1 |
| 26 | FA (6) | RDS3 complex subunit 10 | polymer | 84 | 9888.2 | 1 | UniProt (P0C074) Pfam (PF07189) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Splicing factor 3b subunit |
| 27 | GA (L) | Pre-mRNA-splicing factor CEF1 | polymer | 590 | 67837.8 | 1 | UniProt (Q03654) Pfam (PF00249) Pfam (PF11831) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PRP nineteen-associated complex protein 85,PRP19-associated complex protein 85 |
| 28 | HA, IA, JA, KA (s, t, q, r) | Pre-mRNA-processing factor 19 | polymer | 503 | 56629.8 | 4 | UniProt (A0A6A5PQI0) | Saccharomyces cerevisiae (Baker's yeast) | |
| 29 | LA (K) | SNT309 isoform 1 | polymer | 175 | 20741.5 | 1 | UniProt (A0A6A5Q641) | Saccharomyces cerevisiae (Baker's yeast) | Y55_G0056570.mRNA.1.CDS.1 |
| 30 | MA (N) | BUD31 isoform 1 | polymer | 157 | 18484.5 | 1 | UniProt (A0A6A5Q3N1) | Saccharomyces cerevisiae (Baker's yeast) | EM14S01-3B_G0005920.mRNA.1.CDS.1,HLJ1_G0006010.mRNA.1.CDS.1,JXXY16.1_G0005970.mRNA.1.CDS.1,Pre-mRNA-splicing factor BUD31,SX2_G0005960.mRNA.1.CDS.1,XXYS1_G0005950.mRNA.1.CDS.1,Y55_G0006020.mRNA.1.CDS.1 |
| 31 | NA (T) | HLJ1_G0054350.mRNA.1.CDS.1 | polymer | 337 | 37639.7 | 1 | UniProt (A0A6L0ZMZ1) | Saccharomyces cerevisiae (Baker's yeast) | Pre-mRNA-splicing factor PRP46 |
| 32 | OA (P) | Pre-mRNA-processing protein 45 | polymer | 379 | 42548.7 | 1 | UniProt (A0A6A5PTY9) | Saccharomyces cerevisiae (Baker's yeast) | |
| 33 | PA (Q) | Pre-mRNA-splicing factor SLT11 | polymer | 364 | 40988.6 | 1 | UniProt (A0A6A5Q0Z2) | Saccharomyces cerevisiae (Baker's yeast) | |
| 34 | QA (R) | Pre-mRNA-splicing factor CWC2 | polymer | 261 | 29742.8 | 1 | UniProt (A0A6A5Q155) | Saccharomyces cerevisiae (Baker's yeast) | |
| 35 | RA (S) | Pre-mRNA-splicing factor CWC15 | polymer | 175 | 19975.2 | 1 | UniProt (Q03772) Pfam (PF04889) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Complexed with CEF1 protein 15 |
| 36 | SA (Y) | Pre-mRNA leakage protein 1 | polymer | 204 | 23685.7 | 1 | UniProt (Q07930) Pfam (PF00498) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 37 | TA (X) | SX2_G0027210.mRNA.1.CDS.1 | polymer | 128 | 14880.5 | 1 | UniProt (A0A6L1ALU2) | Saccharomyces cerevisiae (Baker's yeast) | |
| 38 | UA (Z) | Pre-mRNA-splicing factor CWC26 | polymer | 121 | 14039.6 | 1 | UniProt (A0A6L1AYM3) | Saccharomyces cerevisiae (Baker's yeast) | |
| 39 | VA (W) | CDC40 isoform 1 | polymer | 455 | 52128.8 | 1 | UniProt (A0A6A5PYD5) | Saccharomyces cerevisiae (Baker's yeast) | |
| 40 | WA (U) | Pre-mRNA-splicing factor CWC21 | polymer | 135 | 15793.6 | 1 | UniProt (A0A6A5PZG6) | Saccharomyces cerevisiae (Baker's yeast) | |
| 41 | XA (V) | CWC22 isoform 1 | polymer | 577 | 67386.1 | 1 | UniProt (A0A6A5Q1E9) | Saccharomyces cerevisiae (Baker's yeast) | |
| 42 | YA (M) | Pre-mRNA-splicing factor CWC24 | polymer | 259 | 29797.9 | 1 | UniProt (A0A6A5PVM6) | Saccharomyces cerevisiae (Baker's yeast) | |
| 43 | ZA (z) | CWC27 isoform 1 | polymer | 301 | 35080.9 | 1 | UniProt (A0A6A5Q291) | Saccharomyces cerevisiae (Baker's yeast) | |
| 44 | AB (y) | Pre-mRNA-splicing factor SPP2 | polymer | 185 | 20685.4 | 1 | UniProt (Q02521) Pfam (PF12656) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Spliceosome maturation protein SPP2,Suppressor of PRP protein 2 |
| 45 | BB (J) | CLF1 isoform 1 | polymer | 687 | 82555.9 | 1 | UniProt (A0A6A5PT67) | Saccharomyces cerevisiae (Baker's yeast) | Pre-mRNA-splicing factor CLF1 |
| 46 | CB (I) | SYF1 isoform 1 | polymer | 859 | 100344.0 | 1 | UniProt (A0A6A5Q0A4) | Saccharomyces cerevisiae (Baker's yeast) | |
| 47 | DB (x) | Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2 | polymer | 876 | 99947.5 | 1 | UniProt (P20095) Pfam (PF00270) Pfam (PF00271) Pfam (PF04408) Pfam (PF21010) Pfam (PF07717) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Pre-mRNA-processing protein 2 |
| 48 | EB (A) | INOSITOL HEXAKISPHOSPHATE | non-polymer | 660.0 | 1 | Chemie (IHP) | |||
| 49 | FB (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 50 | GB, IB, JB, KB, LB... (C, F, 3) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) | |||
| 51 | HB (C) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 52 | AC, BC, MB, NB, OB... (M, u, v, 5, N...) | ZINC ION | non-polymer | 65.4 | 15 | Chemie (ZN) | |||
| 53 | CC, DC, EC, FC, GC... (A, B, C, D, F...) | water | water | 18.0 | 1714 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 56 |
| Total formula weight | 2911969.6 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 2350.3 | |
| All* | Total formula weight | 2914319.9 |
