7DCO
Cryo-EM structure of the activated spliceosome (Bact complex) at an atomic resolution of 2.5 angstrom
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | PRP8 isoform 1 | polymer | 2413 | 279867.5 | 1 | UniProt (A0A6A5PW68) | Saccharomyces cerevisiae (Baker's yeast) | |
| 2 | B (B) | U5 snRNA | polymer | 214 | 68643.3 | 1 | GenBank (1039023403) | Saccharomyces cerevisiae | |
| 3 | C (C) | SNU114 isoform 1 | polymer | 1008 | 114174.0 | 1 | UniProt (A0A6A5PW35) | Saccharomyces cerevisiae (Baker's yeast) | |
| 4 | D (D) | BRR2 isoform 1 | polymer | 2163 | 246470.3 | 1 | UniProt (A0A6A5Q2I8) | Saccharomyces cerevisiae (Baker's yeast) | |
| 5 | E (d) | Small nuclear ribonucleoprotein Sm D3 | polymer | 101 | 11240.1 | 1 | UniProt (A0A6A5Q3H8) | Saccharomyces cerevisiae (Baker's yeast) | Sm-D3,snRNP core protein D3 |
| 6 | F, Q (a, h) | BJ4_G0014900.mRNA.1.CDS.1 | polymer | 196 | 22427.0 | 2 | UniProt (A0A6A5Q0I3) | Saccharomyces cerevisiae (Baker's yeast) | HLJ1_G0014660.mRNA.1.CDS.1,SMB1 isoform 1,Y55_G0014800.mRNA.1.CDS.1 |
| 7 | G, V (b, m) | SMD1 isoform 1 | polymer | 146 | 16296.8 | 2 | UniProt (A0A6A5Q0W7) | Saccharomyces cerevisiae (Baker's yeast) | |
| 8 | H, W (c, n) | BJ4_G0037700.mRNA.1.CDS.1 | polymer | 110 | 12876.1 | 2 | UniProt (A0A6L0ZK95) | Saccharomyces cerevisiae (Baker's yeast) | HLJ1_G0037800.mRNA.1.CDS.1,HN1_G0037500.mRNA.1.CDS.1,Y55_G0037640.mRNA.1.CDS.1 |
| 9 | I, R (e, i) | Small nuclear ribonucleoprotein E | polymer | 94 | 10385.1 | 2 | UniProt (A0A6A5Q895) | Saccharomyces cerevisiae (Baker's yeast) | snRNP-E,Sm protein E |
| 10 | J, S (f, j) | Sm protein F | polymer | 86 | 9669.9 | 2 | UniProt (A0A6A5Q5K4) | Saccharomyces cerevisiae (Baker's yeast) | |
| 11 | K, T (g, k) | Small nuclear ribonucleoprotein G | polymer | 77 | 8490.8 | 2 | UniProt (P40204) Pfam (PF01423) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | snRNP-G,Sm protein G,SmG |
| 12 | L (F) | U6 snRNA | polymer | 112 | 35883.2 | 1 | GenBank (1039022925) | Saccharomyces cerevisiae | |
| 13 | M (G) | pre-mRNA | polymer | 162 | 44772.2 | 1 | Saccharomyces cerevisiae | ||
| 14 | N (H) | U2 snRNA | polymer | 1175 | 376324.6 | 1 | Saccharomyces cerevisiae | ||
| 15 | O (o) | HLJ1_G0053790.mRNA.1.CDS.1 | polymer | 238 | 27232.3 | 1 | UniProt (A0A6A5Q2G3) | Saccharomyces cerevisiae (Baker's yeast) | LEA1 isoform 1,Y55_G0053650.mRNA.1.CDS.1 |
| 16 | P (p) | BJ4_G0027490.mRNA.1.CDS.1 | polymer | 111 | 12850.9 | 1 | UniProt (A0A6A5Q318) | Saccharomyces cerevisiae (Baker's yeast) | HN1_G0027200.mRNA.1.CDS.1,MSL1 isoform 1,SX2_G0029270.mRNA.1.CDS.1,Y55_G0027550.mRNA.1.CDS.1 |
| 17 | U (l) | Small nuclear ribonucleoprotein Sm D3 | polymer | 81 | 8882.2 | 1 | UniProt (A0A6A5Q3H8) | Saccharomyces cerevisiae (Baker's yeast) | Sm-D3,snRNP core protein D3 |
| 18 | X (u) | PRP9 isoform 1 | polymer | 530 | 63126.4 | 1 | UniProt (A0A6A5PZ67) | Saccharomyces cerevisiae (Baker's yeast) | |
| 19 | Y (w) | Pre-mRNA-splicing factor PRP21 | polymer | 280 | 33111.5 | 1 | UniProt (P32524) Pfam (PF12230) Pfam (PF01805) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 20 | Z (v) | PRP11 isoform 1 | polymer | 266 | 29962.8 | 1 | UniProt (A0A6A5Q100) | Saccharomyces cerevisiae (Baker's yeast) | |
| 21 | AA (1) | HSH155 isoform 1 | polymer | 971 | 110166.7 | 1 | UniProt (A0A6A5PSM1) | Saccharomyces cerevisiae (Baker's yeast) | |
| 22 | BA (2) | HLJ1_G0043010.mRNA.1.CDS.1 | polymer | 238 | 27503.8 | 1 | UniProt (A0A6L0ZSI9) | Saccharomyces cerevisiae (Baker's yeast) | |
| 23 | CA (3) | RSE1 isoform 1 | polymer | 1361 | 153956.8 | 1 | UniProt (A0A6A5PUP5) | Saccharomyces cerevisiae (Baker's yeast) | |
| 24 | DA (4) | HSH49 isoform 1 | polymer | 213 | 24534.2 | 1 | UniProt (A0A6A5Q466) | Saccharomyces cerevisiae (Baker's yeast) | |
| 25 | EA (5) | BJ4_G0056610.mRNA.1.CDS.1 | polymer | 107 | 12283.6 | 1 | UniProt (A0A6A5Q1K7) | Saccharomyces cerevisiae (Baker's yeast) | EM14S01-3B_G0056430.mRNA.1.CDS.1,HLJ1_G0056610.mRNA.1.CDS.1,HN1_G0056420.mRNA.1.CDS.1,JXXY16.1_G0056310.mRNA.1.CDS.1,RDS3 isoform 1,SX2_G0056170.mRNA.1.CDS.1,XXYS1_G0055940.mRNA.1.CDS.1,Y55_G0056510.mRNA.1.CDS.1 |
| 26 | FA (6) | RDS3 complex subunit 10 | polymer | 84 | 9888.2 | 1 | UniProt (P0C074) Pfam (PF07189) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Splicing factor 3b subunit |
| 27 | GA (L) | Pre-mRNA-splicing factor CEF1 | polymer | 590 | 67837.8 | 1 | UniProt (Q03654) Pfam (PF00249) Pfam (PF11831) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PRP nineteen-associated complex protein 85,PRP19-associated complex protein 85 |
| 28 | HA, IA, JA, KA (s, t, q, r) | Pre-mRNA-processing factor 19 | polymer | 503 | 56629.8 | 4 | UniProt (A0A6A5PQI0) | Saccharomyces cerevisiae (Baker's yeast) | |
| 29 | LA (K) | SNT309 isoform 1 | polymer | 175 | 20741.5 | 1 | UniProt (A0A6A5Q641) | Saccharomyces cerevisiae (Baker's yeast) | Y55_G0056570.mRNA.1.CDS.1 |
| 30 | MA (N) | BUD31 isoform 1 | polymer | 157 | 18484.5 | 1 | UniProt (A0A6A5Q3N1) | Saccharomyces cerevisiae (Baker's yeast) | EM14S01-3B_G0005920.mRNA.1.CDS.1,HLJ1_G0006010.mRNA.1.CDS.1,JXXY16.1_G0005970.mRNA.1.CDS.1,Pre-mRNA-splicing factor BUD31,SX2_G0005960.mRNA.1.CDS.1,XXYS1_G0005950.mRNA.1.CDS.1,Y55_G0006020.mRNA.1.CDS.1 |
| 31 | NA (T) | HLJ1_G0054350.mRNA.1.CDS.1 | polymer | 337 | 37639.7 | 1 | UniProt (A0A6L0ZMZ1) | Saccharomyces cerevisiae (Baker's yeast) | Pre-mRNA-splicing factor PRP46 |
| 32 | OA (P) | Pre-mRNA-processing protein 45 | polymer | 379 | 42548.7 | 1 | UniProt (A0A6A5PTY9) | Saccharomyces cerevisiae (Baker's yeast) | |
| 33 | PA (Q) | Pre-mRNA-splicing factor SLT11 | polymer | 364 | 40988.6 | 1 | UniProt (A0A6A5Q0Z2) | Saccharomyces cerevisiae (Baker's yeast) | |
| 34 | QA (R) | Pre-mRNA-splicing factor CWC2 | polymer | 261 | 29742.8 | 1 | UniProt (A0A6A5Q155) | Saccharomyces cerevisiae (Baker's yeast) | |
| 35 | RA (S) | Pre-mRNA-splicing factor CWC15 | polymer | 175 | 19975.2 | 1 | UniProt (Q03772) Pfam (PF04889) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Complexed with CEF1 protein 15 |
| 36 | SA (Y) | Pre-mRNA leakage protein 1 | polymer | 204 | 23685.7 | 1 | UniProt (Q07930) Pfam (PF00498) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 37 | TA (X) | SX2_G0027210.mRNA.1.CDS.1 | polymer | 128 | 14880.5 | 1 | UniProt (A0A6L1ALU2) | Saccharomyces cerevisiae (Baker's yeast) | |
| 38 | UA (Z) | Pre-mRNA-splicing factor CWC26 | polymer | 121 | 14039.6 | 1 | UniProt (A0A6L1AYM3) | Saccharomyces cerevisiae (Baker's yeast) | |
| 39 | VA (W) | CDC40 isoform 1 | polymer | 455 | 52128.8 | 1 | UniProt (A0A6A5PYD5) | Saccharomyces cerevisiae (Baker's yeast) | |
| 40 | WA (U) | Pre-mRNA-splicing factor CWC21 | polymer | 135 | 15793.6 | 1 | UniProt (A0A6A5PZG6) | Saccharomyces cerevisiae (Baker's yeast) | |
| 41 | XA (V) | CWC22 isoform 1 | polymer | 577 | 67386.1 | 1 | UniProt (A0A6A5Q1E9) | Saccharomyces cerevisiae (Baker's yeast) | |
| 42 | YA (M) | Pre-mRNA-splicing factor CWC24 | polymer | 259 | 29797.9 | 1 | UniProt (A0A6A5PVM6) | Saccharomyces cerevisiae (Baker's yeast) | |
| 43 | ZA (z) | CWC27 isoform 1 | polymer | 301 | 35080.9 | 1 | UniProt (A0A6A5Q291) | Saccharomyces cerevisiae (Baker's yeast) | |
| 44 | AB (y) | Pre-mRNA-splicing factor SPP2 | polymer | 185 | 20685.4 | 1 | UniProt (Q02521) Pfam (PF12656) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Spliceosome maturation protein SPP2,Suppressor of PRP protein 2 |
| 45 | BB (J) | CLF1 isoform 1 | polymer | 687 | 82555.9 | 1 | UniProt (A0A6A5PT67) | Saccharomyces cerevisiae (Baker's yeast) | Pre-mRNA-splicing factor CLF1 |
| 46 | CB (I) | SYF1 isoform 1 | polymer | 859 | 100344.0 | 1 | UniProt (A0A6A5Q0A4) | Saccharomyces cerevisiae (Baker's yeast) | |
| 47 | DB (x) | Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2 | polymer | 876 | 99947.5 | 1 | UniProt (P20095) Pfam (PF00270) Pfam (PF00271) Pfam (PF04408) Pfam (PF21010) Pfam (PF07717) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Pre-mRNA-processing protein 2 |
| 48 | EB (A) | INOSITOL HEXAKISPHOSPHATE | non-polymer | 660.0 | 1 | Chemie (IHP) | |||
| 49 | FB (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 50 | GB, IB, JB, KB, LB... (C, F, 3) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) PubChem (888) | |||
| 51 | HB (C) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) PubChem (271) | |||
| 52 | AC, BC, MB, NB, OB... (M, u, v, 5, N...) | ZINC ION | non-polymer | 65.4 | 15 | Chemie (ZN) | |||
| 53 | CC, DC, EC, FC, GC... (A, B, C, D, F...) | water | water | 18.0 | 1714 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 56 |
| Total formula weight | 2911969.6 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 2350.3 | |
| All* | Total formula weight | 2914319.9 |
