7DCO

Cryo-EM structure of the activated spliceosome (Bact complex) at an atomic resolution of 2.5 angstrom

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
1	0000245	biological_process	spliceosomal complex assembly
1	0003729	molecular_function	mRNA binding
2	0005634	cellular_component	nucleus
3	0003676	molecular_function	nucleic acid binding
3	0005634	cellular_component	nucleus
4	0003676	molecular_function	nucleic acid binding
4	0003723	molecular_function	RNA binding
5	0000398	biological_process	mRNA splicing, via spliceosome
6	0000245	biological_process	spliceosomal complex assembly
6	0000398	biological_process	mRNA splicing, via spliceosome
6	0003674	molecular_function	molecular_function
6	0005515	molecular_function	protein binding
6	0005634	cellular_component	nucleus
6	0005686	cellular_component	U2 snRNP
6	0006397	biological_process	mRNA processing
6	0008380	biological_process	RNA splicing
6	0071011	cellular_component	precatalytic spliceosome
6	1903241	biological_process	U2-type prespliceosome assembly
a	0003723	molecular_function	RNA binding
A	0000398	biological_process	mRNA splicing, via spliceosome
A	0003723	molecular_function	RNA binding
A	0005681	cellular_component	spliceosomal complex
A	0008233	molecular_function	peptidase activity
A	0008237	molecular_function	metallopeptidase activity
A	0017070	molecular_function	U6 snRNA binding
A	0030623	molecular_function	U5 snRNA binding
b	0003723	molecular_function	RNA binding
b	0006396	biological_process	RNA processing
c	0003723	molecular_function	RNA binding
c	0008380	biological_process	RNA splicing
c	0030532	cellular_component	small nuclear ribonucleoprotein complex
C	0003924	molecular_function	GTPase activity
C	0005525	molecular_function	GTP binding
d	0000387	biological_process	spliceosomal snRNP assembly
d	0003723	molecular_function	RNA binding
d	0005681	cellular_component	spliceosomal complex
d	0006396	biological_process	RNA processing
D	0003676	molecular_function	nucleic acid binding
D	0005524	molecular_function	ATP binding
e	0000398	biological_process	mRNA splicing, via spliceosome
e	0003723	molecular_function	RNA binding
e	0005681	cellular_component	spliceosomal complex
f	0000398	biological_process	mRNA splicing, via spliceosome
f	0003723	molecular_function	RNA binding
f	0005681	cellular_component	spliceosomal complex
g	0000245	biological_process	spliceosomal complex assembly
g	0000387	biological_process	spliceosomal snRNP assembly
g	0000395	biological_process	mRNA 5'-splice site recognition
g	0000398	biological_process	mRNA splicing, via spliceosome
g	0003674	molecular_function	molecular_function
g	0003723	molecular_function	RNA binding
g	0005515	molecular_function	protein binding
g	0005634	cellular_component	nucleus
g	0005681	cellular_component	spliceosomal complex
g	0005682	cellular_component	U5 snRNP
g	0005685	cellular_component	U1 snRNP
g	0005686	cellular_component	U2 snRNP
g	0005687	cellular_component	U4 snRNP
g	0005737	cellular_component	cytoplasm
g	0006397	biological_process	mRNA processing
g	0008150	biological_process	biological_process
g	0008380	biological_process	RNA splicing
g	0032991	cellular_component	protein-containing complex
g	0034719	cellular_component	SMN-Sm protein complex
g	0036261	biological_process	7-methylguanosine cap hypermethylation
g	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
g	0071004	cellular_component	U2-type prespliceosome
g	0071011	cellular_component	precatalytic spliceosome
g	0071013	cellular_component	catalytic step 2 spliceosome
g	0097526	cellular_component	spliceosomal tri-snRNP complex
g	1903241	biological_process	U2-type prespliceosome assembly
g	1990904	cellular_component	ribonucleoprotein complex
h	0003723	molecular_function	RNA binding
i	0000398	biological_process	mRNA splicing, via spliceosome
i	0003723	molecular_function	RNA binding
i	0005681	cellular_component	spliceosomal complex
I	0000398	biological_process	mRNA splicing, via spliceosome
I	0006396	biological_process	RNA processing
j	0000398	biological_process	mRNA splicing, via spliceosome
j	0003723	molecular_function	RNA binding
j	0005681	cellular_component	spliceosomal complex
J	0000398	biological_process	mRNA splicing, via spliceosome
J	0006396	biological_process	RNA processing
k	0000245	biological_process	spliceosomal complex assembly
k	0000387	biological_process	spliceosomal snRNP assembly
k	0000395	biological_process	mRNA 5'-splice site recognition
k	0000398	biological_process	mRNA splicing, via spliceosome
k	0003674	molecular_function	molecular_function
k	0003723	molecular_function	RNA binding
k	0005515	molecular_function	protein binding
k	0005634	cellular_component	nucleus
k	0005681	cellular_component	spliceosomal complex
k	0005682	cellular_component	U5 snRNP
k	0005685	cellular_component	U1 snRNP
k	0005686	cellular_component	U2 snRNP
k	0005687	cellular_component	U4 snRNP
k	0005737	cellular_component	cytoplasm
k	0006397	biological_process	mRNA processing
k	0008150	biological_process	biological_process
k	0008380	biological_process	RNA splicing
k	0032991	cellular_component	protein-containing complex
k	0034719	cellular_component	SMN-Sm protein complex
k	0036261	biological_process	7-methylguanosine cap hypermethylation
k	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
k	0071004	cellular_component	U2-type prespliceosome
k	0071011	cellular_component	precatalytic spliceosome
k	0071013	cellular_component	catalytic step 2 spliceosome
k	0097526	cellular_component	spliceosomal tri-snRNP complex
k	1903241	biological_process	U2-type prespliceosome assembly
k	1990904	cellular_component	ribonucleoprotein complex
l	0000387	biological_process	spliceosomal snRNP assembly
l	0003723	molecular_function	RNA binding
l	0005681	cellular_component	spliceosomal complex
l	0006396	biological_process	RNA processing
L	0000350	biological_process	generation of catalytic spliceosome for second transesterification step
L	0000386	molecular_function	second spliceosomal transesterification activity
L	0000398	biological_process	mRNA splicing, via spliceosome
L	0000974	cellular_component	Prp19 complex
L	0003677	molecular_function	DNA binding
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005681	cellular_component	spliceosomal complex
L	0005737	cellular_component	cytoplasm
L	0006397	biological_process	mRNA processing
L	0008380	biological_process	RNA splicing
L	0071006	cellular_component	U2-type catalytic step 1 spliceosome
m	0003723	molecular_function	RNA binding
m	0006396	biological_process	RNA processing
M	0046872	molecular_function	metal ion binding
n	0003723	molecular_function	RNA binding
n	0008380	biological_process	RNA splicing
n	0030532	cellular_component	small nuclear ribonucleoprotein complex
N	0005634	cellular_component	nucleus
o	0000398	biological_process	mRNA splicing, via spliceosome
o	0030620	molecular_function	U2 snRNA binding
p	0003676	molecular_function	nucleic acid binding
p	0003723	molecular_function	RNA binding
P	0000398	biological_process	mRNA splicing, via spliceosome
P	0005681	cellular_component	spliceosomal complex
q	0000398	biological_process	mRNA splicing, via spliceosome
q	0000974	cellular_component	Prp19 complex
q	0004842	molecular_function	ubiquitin-protein transferase activity
q	0016567	biological_process	protein ubiquitination
q	0061630	molecular_function	ubiquitin protein ligase activity
Q	0003723	molecular_function	RNA binding
r	0000398	biological_process	mRNA splicing, via spliceosome
r	0000974	cellular_component	Prp19 complex
r	0004842	molecular_function	ubiquitin-protein transferase activity
r	0016567	biological_process	protein ubiquitination
r	0061630	molecular_function	ubiquitin protein ligase activity
R	0003676	molecular_function	nucleic acid binding
R	0003723	molecular_function	RNA binding
R	0046872	molecular_function	metal ion binding
s	0000398	biological_process	mRNA splicing, via spliceosome
s	0000974	cellular_component	Prp19 complex
s	0004842	molecular_function	ubiquitin-protein transferase activity
s	0016567	biological_process	protein ubiquitination
s	0061630	molecular_function	ubiquitin protein ligase activity
S	0000398	biological_process	mRNA splicing, via spliceosome
S	0003674	molecular_function	molecular_function
S	0003723	molecular_function	RNA binding
S	0005515	molecular_function	protein binding
S	0005634	cellular_component	nucleus
S	0005681	cellular_component	spliceosomal complex
S	0005684	cellular_component	U2-type spliceosomal complex
S	0006397	biological_process	mRNA processing
S	0008380	biological_process	RNA splicing
S	0045292	biological_process	mRNA cis splicing, via spliceosome
S	0071013	cellular_component	catalytic step 2 spliceosome
t	0000398	biological_process	mRNA splicing, via spliceosome
t	0000974	cellular_component	Prp19 complex
t	0004842	molecular_function	ubiquitin-protein transferase activity
t	0016567	biological_process	protein ubiquitination
t	0061630	molecular_function	ubiquitin protein ligase activity
T	0000398	biological_process	mRNA splicing, via spliceosome
u	0000398	biological_process	mRNA splicing, via spliceosome
u	0003676	molecular_function	nucleic acid binding
u	0003723	molecular_function	RNA binding
u	0005634	cellular_component	nucleus
u	0005681	cellular_component	spliceosomal complex
u	0008270	molecular_function	zinc ion binding
v	0003676	molecular_function	nucleic acid binding
v	0008270	molecular_function	zinc ion binding
V	0003723	molecular_function	RNA binding
w	0000398	biological_process	mRNA splicing, via spliceosome
w	0003723	molecular_function	RNA binding
w	0005515	molecular_function	protein binding
w	0005634	cellular_component	nucleus
w	0005681	cellular_component	spliceosomal complex
w	0005686	cellular_component	U2 snRNP
w	0006396	biological_process	RNA processing
w	0006397	biological_process	mRNA processing
w	0008380	biological_process	RNA splicing
w	0045292	biological_process	mRNA cis splicing, via spliceosome
w	0071004	cellular_component	U2-type prespliceosome
w	0071013	cellular_component	catalytic step 2 spliceosome
w	1903241	biological_process	U2-type prespliceosome assembly
W	0000398	biological_process	mRNA splicing, via spliceosome
W	0071013	cellular_component	catalytic step 2 spliceosome
x	0000166	molecular_function	nucleotide binding
x	0000349	biological_process	generation of catalytic spliceosome for first transesterification step
x	0000398	biological_process	mRNA splicing, via spliceosome
x	0003676	molecular_function	nucleic acid binding
x	0003723	molecular_function	RNA binding
x	0003724	molecular_function	RNA helicase activity
x	0004386	molecular_function	helicase activity
x	0005515	molecular_function	protein binding
x	0005524	molecular_function	ATP binding
x	0005634	cellular_component	nucleus
x	0006397	biological_process	mRNA processing
x	0008186	molecular_function	ATP-dependent activity, acting on RNA
x	0008380	biological_process	RNA splicing
x	0016787	molecular_function	hydrolase activity
x	0016887	molecular_function	ATP hydrolysis activity
x	0022613	biological_process	ribonucleoprotein complex biogenesis
x	0034247	biological_process	snoRNA splicing
x	0071006	cellular_component	U2-type catalytic step 1 spliceosome
x	0071826	biological_process	protein-RNA complex organization
X	0000398	biological_process	mRNA splicing, via spliceosome
X	0003676	molecular_function	nucleic acid binding
X	0003723	molecular_function	RNA binding
y	0000398	biological_process	mRNA splicing, via spliceosome
y	0001671	molecular_function	ATPase activator activity
y	0003676	molecular_function	nucleic acid binding
y	0005634	cellular_component	nucleus
y	0005681	cellular_component	spliceosomal complex
y	0005737	cellular_component	cytoplasm
y	0006397	biological_process	mRNA processing
y	0008380	biological_process	RNA splicing
Y	0000398	biological_process	mRNA splicing, via spliceosome
Y	0003674	molecular_function	molecular_function
Y	0003729	molecular_function	mRNA binding
Y	0005515	molecular_function	protein binding
Y	0005634	cellular_component	nucleus
Y	0005737	cellular_component	cytoplasm
Y	0006397	biological_process	mRNA processing
Y	0006406	biological_process	mRNA export from nucleus
Y	0008380	biological_process	RNA splicing
Y	0051237	biological_process	maintenance of RNA location
Y	0070274	cellular_component	RES complex
Y	1903241	biological_process	U2-type prespliceosome assembly
z	0000413	biological_process	protein peptidyl-prolyl isomerization
z	0003755	molecular_function	peptidyl-prolyl cis-trans isomerase activity

Functional Information from PROSITE/UniProt

site_id	PS00012
Number of Residues	16
Details	PHOSPHOPANTETHEINE Phosphopantetheine attachment site. GINIDSLKFNELVKLM

Chain	Residue	Details
D	GLY174-MET189

---

site_id	PS00028
Number of Residues	23
Details	ZINC_FINGER_C2H2_1 Zinc finger C2H2 type domain signature. Cpf..CsrwFktssvfesHlvgkiH

Chain	Residue	Details
u	CYS282-HIS304

---

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. IATAgrDsVIKLWDM

Chain	Residue	Details
T	ILE239-MET253
T	VAL281-VAL295
W	ILE178-PHE192

---

site_id	PS00690
Number of Residues	10
Details	DEAH_ATP_HELICASE DEAH-box subfamily ATP-dependent helicases signature. ScIMIDEAHE

Chain	Residue	Details
x	SER341-GLU350

---

site_id	PS00997
Number of Residues	23
Details	G10_1 G10 protein signature 1. LCClRCiqknEtNngstCiCRVP

Chain	Residue	Details
N	LEU103-PRO125

---

site_id	PS00998
Number of Residues	10
Details	G10_2 G10 protein signature 2. CvHCGCRGCA

Chain	Residue	Details
N	CYS145-ALA154

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	40
Details	Repeat: {"description":"SURP motif 2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	47
Details	Domain: {"description":"HTH myb-type 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00625","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	45
Details	DNA binding: {"description":"H-T-H motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU00625","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	19
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	14
Details	Compositional bias: {"description":"Basic and acidic residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	166
Details	Domain: {"description":"Helicase ATP-binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	174
Details	Domain: {"description":"Helicase C-terminal","evidences":[{"source":"PROSITE-ProRule","id":"PRU00542","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	3
Details	Motif: {"description":"DEAH box"}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---