7CLZ
Crystal structure of Alp1U W187F/Y247F in complex with fluostatin C
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Putative hydrolase | polymer | 339 | 36995.9 | 4 | UniProt (Q1RQU8) Pfam (PF00561) UniProt (by SIFTS) (A0A0K2AJY3) In PDB | Streptomyces ambofaciens (strain ATCC 23877 / 3486 / DSM 40053 / JCM 4204 / NBRC 12836 / NRRL B-2516) | Alp1U |
2 | A, C, D | Fluostatin C | non-polymer | 324.3 | 3 | Chemie (DY9) | |||
3 | A, B | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
4 | A, B, D | D-MALATE | non-polymer | 134.1 | 4 | Chemie (MLT) | |||
5 | C, D | BORIC ACID | non-polymer | 61.8 | 2 | Chemie (BO3) | |||
6 | water | water | 18.0 | 354 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 319 (UniProt: Q1RQU8)
PDB | External Database | Details |
---|---|---|
Met -19 | - | expression tag |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Phe 187 | Trp 187 | engineered mutation |
Phe 247 | Tyr 247 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 147983.5 | |
Non-Polymers* | Number of molecules | 11 |
Total formula weight | 1678.8 | |
All* | Total formula weight | 149662.4 |