7CET

Crystal structure of D-cycloserine-bound form of cysteine desulfurase NifS from Helicobacter pylori

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cysteine desulfurase IscS	polymer	401	44125.2	1	UniProt (O25008) Pfam (PF00266)	Helicobacter pylori 26695
2	B (A)	ISOPROPYL ALCOHOL	non-polymer		60.1	1	Chemie (IPA)
3	C (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	D (A)	(5-hydroxy-6-methyl-4-{[(3-oxo-2,3-dihydro-1,2-oxazol-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate	non-polymer		331.2	1	Chemie (7TS)
5	E (A)	water	water		18.0	26	Chemie (HOH)

Sequence modifications

A: 1 - 387 (UniProt: O25008)

PDB	External Database	Details
Val 2	Leu 2	engineered mutation
Arg 138	Lys 138	engineered mutation
Val 388	-	expression tag
Asp 389	-	expression tag
Leu 390	-	expression tag
Val 391	-	expression tag
Pro 392	-	expression tag
Arg 393	-	expression tag
Gly 394	-	expression tag
Ser 395	-	expression tag
His 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	44125.2
Non-Polymers*	Number of molecules	3
	Total formula weight	426.8
All*	Total formula weight	44552.0

*Water molecules are not included.