7C8E

Crystal Structure of 14-3-3 epsilon with 9J10 peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	14-3-3 protein epsilon	polymer	266	30170.3	2	UniProt (P62258) Pfam (PF00244)	Homo sapiens (Human)	14-3-3E
2	C, D (C, D)	9J10	polymer	12	1524.6	2		Streptomyces avermitilis MA-4680 = NBRC 14893
3	E (B)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
4	F, G, H, I (A, B, C, D)	water	water		18.0	29	Chemie (HOH)

Sequence modifications

A, B: 1 - 232 (UniProt: P62258)

PDB	External Database	Details
Met -33	-	initiating methionine
Gly -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Pro -18	-	expression tag
Arg -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	63389.7
Non-Polymers*	Number of molecules	1
	Total formula weight	122.1
All*	Total formula weight	63511.9

*Water molecules are not included.