7C8E
Crystal Structure of 14-3-3 epsilon with 9J10 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-01-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.470, 83.350, 118.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.160 |
| R-factor | 0.2242 |
| Rwork | 0.222 |
| R-free | 0.27050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2br9 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.241 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.234 | 3.330 |
| High resolution limit [Å] | 3.160 | 3.160 |
| Rmerge | 0.645 | |
| Rmeas | 0.163 | 0.729 |
| Rpim | 0.075 | 0.335 |
| Number of reflections | 10688 | 1516 |
| <I/σ(I)> | 8.6 | 2.3 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 4.6 | 4.6 |
| CC(1/2) | 0.995 | 0.698 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289.15 | 28% PEG 400, 0.1M Hepes pH 7.5, 0.2M Calcium chloride |
