7C5O

Crystal Structure of H177A mutant of Glyceraldehyde-3-phosphate-dehydrogenase1 from Escherichia coli complexed with NAD at 1.98 Angstrom resolution.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (O, P, Q, R)	Glyceraldehyde-3-phosphate dehydrogenase	polymer	352	37820.9	4	UniProt (A0A140NCK4)	Escherichia coli BL21(DE3)
2	E, I, K, M (O, P, Q, R)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
3	F (O)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
4	G, H, J, L, N (O, P, Q, R)	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
5	O, P, Q, R (O, P, Q, R)	water	water		18.0	1080	Chemie (HOH)

Sequence modifications

O, P, Q, R: 1 - 333 (UniProt: A0A140NCK4)

PDB	External Database	Details
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ala 177	His 177	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	151283.4
Non-Polymers*	Number of molecules	10
	Total formula weight	2925.9
All*	Total formula weight	154209.3

*Water molecules are not included.