7C5O
Crystal Structure of H177A mutant of Glyceraldehyde-3-phosphate-dehydrogenase1 from Escherichia coli complexed with NAD at 1.98 Angstrom resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 89.972, 89.972, 342.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.175 - 1.981 |
| R-factor | 0.1486 |
| Rwork | 0.146 |
| R-free | 0.19140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7c5f |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.075 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.179 | 0.489 |
| Number of reflections | 98318 | 98318 |
| <I/σ(I)> | 21.3 | 6.75 |
| Completeness [%] | 99.1 | 97.4 |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM sodium Cacodylate pH 6.5, 18%(w/v)PEG 1000, 200mM MgCl2 |
