7C5O

Crystal Structure of H177A mutant of Glyceraldehyde-3-phosphate-dehydrogenase1 from Escherichia coli complexed with NAD at 1.98 Angstrom resolution.

Functional Information from GO Data

Chain	GOid	namespace	contents
O	0000166	molecular_function	nucleotide binding
O	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
O	0005737	cellular_component	cytoplasm
O	0006006	biological_process	glucose metabolic process
O	0016491	molecular_function	oxidoreductase activity
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0042802	molecular_function	identical protein binding
O	0050661	molecular_function	NADP binding
O	0051287	molecular_function	NAD binding
P	0000166	molecular_function	nucleotide binding
P	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
P	0005737	cellular_component	cytoplasm
P	0006006	biological_process	glucose metabolic process
P	0016491	molecular_function	oxidoreductase activity
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0042802	molecular_function	identical protein binding
P	0050661	molecular_function	NADP binding
P	0051287	molecular_function	NAD binding
Q	0000166	molecular_function	nucleotide binding
Q	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Q	0005737	cellular_component	cytoplasm
Q	0006006	biological_process	glucose metabolic process
Q	0016491	molecular_function	oxidoreductase activity
Q	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Q	0042802	molecular_function	identical protein binding
Q	0050661	molecular_function	NADP binding
Q	0051287	molecular_function	NAD binding
R	0000166	molecular_function	nucleotide binding
R	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
R	0005737	cellular_component	cytoplasm
R	0006006	biological_process	glucose metabolic process
R	0016491	molecular_function	oxidoreductase activity
R	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
R	0042802	molecular_function	identical protein binding
R	0050661	molecular_function	NADP binding
R	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	31
Details	binding site for residue NAD O 401

Chain	Residue
O	ASN7
O	LYS78
O	CYS96
O	THR97
O	GLY98
O	PHE99
O	SER120
O	ALA121
O	CYS150
O	ASN315
O	PHE319
O	GLY8
O	HOH511
O	HOH514
O	HOH524
O	HOH594
O	HOH633
O	HOH653
O	HOH656
O	HOH667
O	HOH677
O	HOH690
O	PHE9
O	HOH694
O	HOH708
O	GLY10
O	ARG11
O	ILE12
O	ASN33
O	ASP34
O	LEU35

---

site_id	AC2
Number of Residues	6
Details	binding site for residue PO4 O 402

Chain	Residue
O	ALA201
O	HOH574
P	ALA201
Q	ALA201
R	ALA201
R	HOH610

---

site_id	AC3
Number of Residues	3
Details	binding site for residue CL O 403

Chain	Residue
O	LYS213
O	LYS227
O	GLY228

---

site_id	AC4
Number of Residues	6
Details	binding site for residue CL O 404

Chain	Residue
O	ALA80
O	LYS81
O	ALA108
O	HOH582
O	HOH768
O	HOH820

---

site_id	AC5
Number of Residues	31
Details	binding site for residue NAD P 401

Chain	Residue
P	ASN7
P	GLY8
P	PHE9
P	GLY10
P	ARG11
P	ILE12
P	ASN33
P	ASP34
P	LYS78
P	CYS96
P	THR97
P	GLY98
P	PHE99
P	SER120
P	ALA121
P	CYS150
P	ASN315
P	PHE319
P	HOH502
P	HOH508
P	HOH512
P	HOH591
P	HOH602
P	HOH616
P	HOH620
P	HOH628
P	HOH631
P	HOH634
P	HOH653
P	HOH695
Q	HOH562

---

site_id	AC6
Number of Residues	4
Details	binding site for residue CL P 402

Chain	Residue
P	LYS213
P	LYS227
P	GLY228
P	HOH775

---

site_id	AC7
Number of Residues	31
Details	binding site for residue NAD Q 401

Chain	Residue
Q	HOH537
Q	HOH548
Q	HOH567
Q	HOH574
Q	HOH585
Q	HOH607
Q	HOH618
Q	HOH620
Q	HOH633
Q	HOH648
Q	HOH674
Q	HOH678
Q	HOH679
Q	ASN7
Q	GLY8
Q	GLY10
Q	ARG11
Q	ILE12
Q	ASN33
Q	ASP34
Q	LEU35
Q	LYS78
Q	CYS96
Q	THR97
Q	GLY98
Q	PHE99
Q	SER120
Q	ALA121
Q	CYS150
Q	ASN315
Q	PHE319

---

site_id	AC8
Number of Residues	3
Details	binding site for residue CL Q 402

Chain	Residue
Q	LYS227
Q	GLY228
Q	HOH774

---

site_id	AC9
Number of Residues	27
Details	binding site for residue NAD R 401

Chain	Residue
R	GLY8
R	GLY10
R	ARG11
R	ILE12
R	ASP34
R	LEU35
R	LYS78
R	CYS96
R	THR97
R	GLY98
R	PHE99
R	SER120
R	ALA121
R	CYS150
R	ASN315
R	PHE319
R	HOH512
R	HOH520
R	HOH537
R	HOH539
R	HOH544
R	HOH570
R	HOH573
R	HOH584
R	HOH605
R	HOH607
R	HOH632

---

site_id	AD1
Number of Residues	2
Details	binding site for residue CL R 402

Chain	Residue
R	LYS227
R	GLY228

---

Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
O	ALA148-LEU155

---

site_id	PS00430
Number of Residues	89
Details	TONB_DEPENDENT_REC_1 TonB-dependent receptor (TBDR) proteins signature 1. hhhhhhssglvprgshmasmskvgingfgrigrlvlrrllevksnidvvaindltspkilayllkhdsnygpfpwsvdfte..................................DSLIVDGK

Chain	Residue	Details
O	HIS-18-LYS70

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