7C2J

Crystal structure of nsp16-nsp10 heterodimer from SARS-CoV-2 in complex with SAM (without additional SAM during crystallization)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	2'-O-methyltransferase	polymer	305	34315.3	1	UniProt (P0DTD1) Pfam (PF06460)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,Non-structural protein 16,nsp16,Replicase polyprotein 1ab
2	B (B)	Non-structural protein 10	polymer	144	15214.4	1	UniProt (P0DTD1) Pfam (PF09401)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,nsp10,Growth factor-like peptide,GFL,Replicase polyprotein 1ab
3	C (A)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM) PubChem (58766358) PubChem (87224684) PubChem (131697735) PubChem (145712080) PubChem (58633578) PubChem (60038769) PubChem (73897310) PubChem (5136) PubChem (34755) PubChem (9865603) PubChem (12312798) PubChem (132988922) PubChem (134692588) PubChem (135030730) PubChem (140459785) PubChem (163046094)
4	D, E (B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	F, G (A, B)	water	water		18.0	103	Chemie (HOH)

Sequence modifications

A: 1 - 298 (UniProt: P0DTD1)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

B: 1 - 139 (UniProt: P0DTD1)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	49529.7
Non-Polymers*	Number of molecules	3
	Total formula weight	529.3
All*	Total formula weight	50059.0

*Water molecules are not included.