7BVF

Cryo-EM structure of Mycobacterium tuberculosis arabinosyltransferase EmbA-EmbB-AcpM2 in complex with ethambutol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Probable arabinosyltransferase B	polymer	1116	120332.4	1	UniProt (P9WNL7) Pfam (PF17689) Pfam (PF04602) Pfam (PF14896)	Mycobacterium tuberculosis H37Rv
2	B (A)	Probable arabinosyltransferase A	polymer	1102	116789.5	1	UniProt (P9WNL9) Pfam (PF17689) Pfam (PF04602) Pfam (PF14896)	Mycobacterium tuberculosis H37Rv
3	C (P)	Meromycolate extension acyl carrier protein	polymer	99	10743.9	1	UniProt (A0R0B3) Pfam (PF00550)	Mycolicibacterium smegmatis MC2 155	ACP
4	D (B)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
5	E (B)	Ethambutol	non-polymer		204.3	1	Chemie (95E)
6	F (B)	MONO-TRANS, OCTA-CIS DECAPRENYL-PHOSPHATE	non-polymer		779.2	1	Chemie (DSL)
7	G, H (B, A)	CARDIOLIPIN	non-polymer		1464.0	2	Chemie (CDL)

Sequence modifications

B: 1 - 1098 (UniProt: P9WNL7)

PDB	External Database	Details
His 1099	-	expression tag
Leu 1100	-	expression tag
Gly 1101	-	expression tag
Gly 1102	-	expression tag
Ile 1103	-	expression tag
Lys 1104	-	expression tag
Ala 1105	-	expression tag
Phe 1106	-	expression tag
His 1107	-	expression tag
His 1108	-	expression tag
His 1109	-	expression tag
His 1110	-	expression tag
His 1111	-	expression tag
His 1112	-	expression tag
His 1113	-	expression tag
His 1114	-	expression tag
His 1115	-	expression tag
His 1116	-	expression tag

A: 2 - 1094 (UniProt: P9WNL9)

PDB	External Database	Details
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Val 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	247865.8
Non-Polymers*	Number of molecules	5
	Total formula weight	3951.6
All*	Total formula weight	251817.4

*Water molecules are not included.