7BVE

Cryo-EM structure of Mycobacterium smegmatis arabinosyltransferase EmbC2-AcpM2 in complex with ethambutol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Integral membrane indolylacetylinositol arabinosyltransferase EmbC	polymer	1084	116878.9	2	UniProt (I7FMU5) Pfam (PF17689) Pfam (PF04602) Pfam (PF14896) UniProt (by SIFTS) (A0R612) In PDB	Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)
2	C, D	Meromycolate extension acyl carrier protein	polymer	99	10743.9	2	UniProt (A0R0B3) Pfam (PF00550) In PDB	Mycolicibacterium smegmatis MC2 155	ACP
3	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	A, B	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
5	A, B	Ethambutol	non-polymer		204.3	2	Chemie (95E)
6	C, D	N~3~-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-N-(2-sulfanylethyl)-beta-alaninamide	non-polymer		358.3	2	Chemie (PN7)

Sequence modifications

A, B: 1 - 1074 (UniProt: I7FMU5)

PDB	External Database	Details
His 1075	-	expression tag
His 1076	-	expression tag
His 1077	-	expression tag
His 1078	-	expression tag
His 1079	-	expression tag
His 1080	-	expression tag
His 1081	-	expression tag
His 1082	-	expression tag
His 1083	-	expression tag
His 1084	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	255245.5
Non-Polymers*	Number of molecules	8
	Total formula weight	1395.4
All*	Total formula weight	256641.0

*Water molecules are not included.