7BTD

Crystal structure of Rheb Y35N mutant bound to GppNHp

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	GTP-binding protein Rheb	polymer	177	20099.9	1	UniProt (Q15382) Pfam (PF00071) In PDB	Homo sapiens (Human)	Ras homolog enriched in brain
2	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
3	A	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	1	Chemie (GNP)
4		water	water		18.0	105	Chemie (HOH)

Sequence modifications

A: 1 - 169 (UniProt: Q15382)

PDB	External Database	Details
Asn 35	Tyr 35	engineered mutation
Leu 170	-	expression tag
Glu 171	-	expression tag
His 172	-	expression tag
His 173	-	expression tag
His 174	-	expression tag
His 175	-	expression tag
His 176	-	expression tag
His 177	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	20099.9
Non-Polymers*	Number of molecules	2
	Total formula weight	546.5
All*	Total formula weight	20646.4

*Water molecules are not included.