7BTD
Crystal structure of Rheb Y35N mutant bound to GppNHp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 97.637, 102.962, 47.715 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.168 |
Rwork | 0.166 |
R-free | 0.20830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xts |
RMSD bond length | 0.009 |
RMSD bond angle | 1.217 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.010 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.100 | 0.061 | 0.547 |
Rmeas | 0.108 | 0.066 | 0.596 |
Rpim | 0.040 | 0.025 | 0.232 |
Number of reflections | 16650 | 1776 | 1622 |
<I/σ(I)> | 4.6 | ||
Completeness [%] | 99.8 | 99.7 | 99.1 |
Redundancy | 7.1 | 7 | 6.3 |
CC(1/2) | 0.996 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 290 | 0.2 M Ammonium acetate: 0.1 M HEPES, pH 7.5: 25% PEG 3350. |