7BG0

Fusion of MBP and the backbone of the long-acting amylin analog AM833.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, D, E	Maltose/maltodextrin-binding periplasmic protein,Islet amyloid polypeptide	polymer	408	44495.3	4	UniProt (P0AEX9) UniProt (P10997) Pfam (PF01547) Pfam (PF00214) In PDB	Escherichia coli (strain K12)	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Amylin,Diabetes-associated peptide,DAP,Insulinoma amyloid peptide
2	C, F	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)

Sequence modifications

A, B, D, E: 27 - 387 (UniProt: P0AEX9)

PDB	External Database	Details
Met 26	-	initiating methionine
Ala 385	Glu 385	engineered mutation
Ala 388	-	linker
Ala 389	-	linker
Ala 390	-	linker
Gln 391	-	linker
Thr 392	-	linker
Asn 393	-	linker
Ala 394	-	linker
Ala 395	-	linker
Ala 396	-	linker

A, B, D, E: 397 - 433 (UniProt: P10997)

PDB	External Database	Details
Glu 410	Asn 47	engineered mutation
Arg 413	Val 50	engineered mutation
Pro 421	Ala 58	engineered mutation
Pro 424	Ser 61	engineered mutation
Pro 425	Ser 62	engineered mutation
Pro 433	Tyr 70	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	177981.2
Branched	Number of molecules	2
	Total formula weight	684.6
All*	Total formula weight	178665.8

*Water molecules are not included.