7BG0

Fusion of MBP and the backbone of the long-acting amylin analog AM833.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-11
Detector	MAR CCD 165 mm
Wavelength(s)	1.54
Spacegroup name	H 3
Unit cell lengths	179.135, 179.135, 150.208
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.550 - 2.890
R-factor	0.2227
Rwork	0.220
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g7v
RMSD bond length	0.004
RMSD bond angle	0.678
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_3965)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.550	2.998
High resolution limit [Å]	2.890	2.895
Rmerge	0.094	0.581
Rmeas	0.104	0.640
Rpim	0.044	0.268
Number of reflections	40035	3961
<I/σ(I)>	16.7	2.43
Completeness [%]	99.8	98.9
Redundancy	5.7	5.7
CC(1/2)	0.995	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	20mM Tris pH 7.4, 50mM NaCl, 0.02 M magnesium chloride, 0.1 M HEPES pH 7.5, 22 %(w/v) polyacrylic acid 5100 sodium salt