7BFI

A double-histidine mutant of HSP47 slows down client release at low pH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, D, E (A, C, D, B)	Collagen-binding protein	polymer	393	44364.8	4	UniProt (E2RHY7) Pfam (PF00079) UniProt (by SIFTS) (A0A8I3N7V2)	Canis lupus familiaris (Dog)	Serpin H1
2	B, F, G, H, I... (F, H, I, J, E...)	15R8 collagen model peptide	polymer	17	1358.5	6	UniProt (by SIFTS) (Q96A83)	Homo sapiens
3	K, L, M, N, O... (A, C, D, B, H...)	water	water		18.0	104	Chemie (HOH)

Sequence modifications

A, C, D, B: 35 - 418 (UniProt: E2RHY7)

PDB	External Database	Details
Met 34	-	initiating methionine
Asn 273	His 273	engineered mutation
Asn 274	His 274	engineered mutation
Leu 419	-	expression tag
Glu 420	-	expression tag
His 421	-	expression tag
His 422	-	expression tag
His 423	-	expression tag
His 424	-	expression tag
His 425	-	expression tag
His 426	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	185610.4
All*	Total formula weight	185610.4

*Water molecules are not included.