7BFI

A double-histidine mutant of HSP47 slows down client release at low pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-22
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	78.704, 77.406, 122.343
Unit cell angles	90.00, 96.60, 90.00

Refinement procedure

Resolution	78.180 - 2.440
R-factor	0.2182
Rwork	0.217
R-free	0.24820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zha
RMSD bond length	0.003
RMSD bond angle	0.596
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.180	2.590
High resolution limit [Å]	2.440	2.440
Rmerge	0.069	0.874
Rmeas	0.070	0.940
Number of reflections	52937	8413
<I/σ(I)>	11.77
Completeness [%]	96.9	95.7
Redundancy	2.73	2.65
CC(1/2)	0.998	0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	8 % Tacsimate, 18% PEG 3350, 0.1 M HEPES, pH 7.5