7BBF

Crystal structure of ubiquitin charged Ube2N (Ube2N~Ub) in complex with Ube2V2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (H, B, E)	Ubiquitin-conjugating enzyme E2 variant 2	polymer	150	16929.3	3	UniProt (Q15819) Pfam (PF00179)	Homo sapiens (Human)	DDVit 1,Enterocyte differentiation-associated factor 1,EDAF-1,Enterocyte differentiation-promoting factor 1,EDPF-1,MMS2 homolog,Vitamin D3-inducible protein
2	D, E, F (G, D, A)	Ubiquitin-conjugating enzyme E2 N	polymer	153	17182.8	3	UniProt (P61088) Pfam (PF00179)	Homo sapiens (Human)	Bendless-like ubiquitin-conjugating enzyme,E2 ubiquitin-conjugating enzyme N,Ubc13,UbcH13,Ubiquitin carrier protein N,Ubiquitin-protein ligase N
3	G, H, I (I, C, F)	Polyubiquitin-C	polymer	76	8576.8	3	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens (Human)
4	J, K, L, M, N... (H, B, E, G, D...)	water	water		18.0	40	Chemie (HOH)

Sequence modifications

H, B, E: 1 - 145 (UniProt: Q15819)

PDB	External Database	Details
Gly -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

G, D, A: 1 - 152 (UniProt: P61088)

PDB	External Database	Details
Gly 0	-	expression tag
Lys 87	Cys 87	engineered mutation
Ala 92	Lys 92	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	128066.6
All*	Total formula weight	128066.6

*Water molecules are not included.