7BBF

Crystal structure of ubiquitin charged Ube2N (Ube2N~Ub) in complex with Ube2V2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 32
Unit cell lengths	145.840, 145.840, 49.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.740 - 2.540
R-factor	0.2081
Rwork	0.206
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5S
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.740	2.630
High resolution limit [Å]	2.540	2.540
Rmerge	0.054	1.347
Number of reflections	38540	3845
<I/σ(I)>	15.81
Completeness [%]	99.9
Redundancy	5.3
CC(1/2)	0.999	0.497

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.1 M bicine Trizma base pH 8.5, 12.5% PEG 1000, 12.5% PEG 3350, 12.5% MPD, 0.2 % of each Anesthetic alkaloids (lidocaine HCl H2O, procaine HCl, proparacaine HCl, tetracaine HCl)