Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7BBF

Crystal structure of ubiquitin charged Ube2N (Ube2N~Ub) in complex with Ube2V2

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000151	cellular_component	ubiquitin ligase complex
A	0000209	biological_process	protein polyubiquitination
A	0000724	biological_process	double-strand break repair via homologous recombination
A	0000729	biological_process	DNA double-strand break processing
A	0003723	molecular_function	RNA binding
A	0004842	molecular_function	ubiquitin-protein transferase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006281	biological_process	DNA repair
A	0006282	biological_process	regulation of DNA repair
A	0006301	biological_process	postreplication repair
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0016567	biological_process	protein ubiquitination
A	0016740	molecular_function	transferase activity
A	0019787	molecular_function	ubiquitin-like protein transferase activity
A	0031371	cellular_component	ubiquitin conjugating enzyme complex
A	0031372	cellular_component	UBC13-MMS2 complex
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0032991	cellular_component	protein-containing complex
A	0033182	biological_process	obsolete regulation of histone ubiquitination
A	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
A	0043130	molecular_function	ubiquitin binding
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0045739	biological_process	positive regulation of DNA repair
A	0050852	biological_process	T cell receptor signaling pathway
A	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
A	0061631	molecular_function	ubiquitin conjugating enzyme activity
A	0070062	cellular_component	extracellular exosome
A	0070534	biological_process	protein K63-linked ubiquitination
A	0097027	molecular_function	ubiquitin-protein transferase activator activity
A	0140374	biological_process	antiviral innate immune response
A	1902523	biological_process	positive regulation of protein K63-linked ubiquitination
A	1902533	biological_process	positive regulation of intracellular signal transduction
A	1904262	biological_process	negative regulation of TORC1 signaling
A	2000781	biological_process	positive regulation of double-strand break repair
B	0000209	biological_process	protein polyubiquitination
B	0000729	biological_process	DNA double-strand break processing
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0006282	biological_process	regulation of DNA repair
B	0006301	biological_process	postreplication repair
B	0016567	biological_process	protein ubiquitination
B	0031372	cellular_component	UBC13-MMS2 complex
B	0042275	biological_process	error-free postreplication DNA repair
B	0070062	cellular_component	extracellular exosome
B	0070534	biological_process	protein K63-linked ubiquitination
B	1902523	biological_process	positive regulation of protein K63-linked ubiquitination
B	2000781	biological_process	positive regulation of double-strand break repair
D	0000151	cellular_component	ubiquitin ligase complex
D	0000209	biological_process	protein polyubiquitination
D	0000724	biological_process	double-strand break repair via homologous recombination
D	0000729	biological_process	DNA double-strand break processing
D	0003723	molecular_function	RNA binding
D	0004842	molecular_function	ubiquitin-protein transferase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006281	biological_process	DNA repair
D	0006282	biological_process	regulation of DNA repair
D	0006301	biological_process	postreplication repair
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0016567	biological_process	protein ubiquitination
D	0016740	molecular_function	transferase activity
D	0019787	molecular_function	ubiquitin-like protein transferase activity
D	0031371	cellular_component	ubiquitin conjugating enzyme complex
D	0031372	cellular_component	UBC13-MMS2 complex
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0032991	cellular_component	protein-containing complex
D	0033182	biological_process	obsolete regulation of histone ubiquitination
D	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
D	0043130	molecular_function	ubiquitin binding
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0045739	biological_process	positive regulation of DNA repair
D	0050852	biological_process	T cell receptor signaling pathway
D	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
D	0061631	molecular_function	ubiquitin conjugating enzyme activity
D	0070062	cellular_component	extracellular exosome
D	0070534	biological_process	protein K63-linked ubiquitination
D	0097027	molecular_function	ubiquitin-protein transferase activator activity
D	0140374	biological_process	antiviral innate immune response
D	1902523	biological_process	positive regulation of protein K63-linked ubiquitination
D	1902533	biological_process	positive regulation of intracellular signal transduction
D	1904262	biological_process	negative regulation of TORC1 signaling
D	2000781	biological_process	positive regulation of double-strand break repair
E	0000209	biological_process	protein polyubiquitination
E	0000729	biological_process	DNA double-strand break processing
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0006282	biological_process	regulation of DNA repair
E	0006301	biological_process	postreplication repair
E	0016567	biological_process	protein ubiquitination
E	0031372	cellular_component	UBC13-MMS2 complex
E	0042275	biological_process	error-free postreplication DNA repair
E	0070062	cellular_component	extracellular exosome
E	0070534	biological_process	protein K63-linked ubiquitination
E	1902523	biological_process	positive regulation of protein K63-linked ubiquitination
E	2000781	biological_process	positive regulation of double-strand break repair
G	0000151	cellular_component	ubiquitin ligase complex
G	0000209	biological_process	protein polyubiquitination
G	0000724	biological_process	double-strand break repair via homologous recombination
G	0000729	biological_process	DNA double-strand break processing
G	0003723	molecular_function	RNA binding
G	0004842	molecular_function	ubiquitin-protein transferase activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006281	biological_process	DNA repair
G	0006282	biological_process	regulation of DNA repair
G	0006301	biological_process	postreplication repair
G	0006511	biological_process	ubiquitin-dependent protein catabolic process
G	0016567	biological_process	protein ubiquitination
G	0016740	molecular_function	transferase activity
G	0019787	molecular_function	ubiquitin-like protein transferase activity
G	0031371	cellular_component	ubiquitin conjugating enzyme complex
G	0031372	cellular_component	UBC13-MMS2 complex
G	0031625	molecular_function	ubiquitin protein ligase binding
G	0032991	cellular_component	protein-containing complex
G	0033182	biological_process	obsolete regulation of histone ubiquitination
G	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
G	0043130	molecular_function	ubiquitin binding
G	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
G	0045739	biological_process	positive regulation of DNA repair
G	0050852	biological_process	T cell receptor signaling pathway
G	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
G	0061631	molecular_function	ubiquitin conjugating enzyme activity
G	0070062	cellular_component	extracellular exosome
G	0070534	biological_process	protein K63-linked ubiquitination
G	0097027	molecular_function	ubiquitin-protein transferase activator activity
G	0140374	biological_process	antiviral innate immune response
G	1902523	biological_process	positive regulation of protein K63-linked ubiquitination
G	1902533	biological_process	positive regulation of intracellular signal transduction
G	1904262	biological_process	negative regulation of TORC1 signaling
G	2000781	biological_process	positive regulation of double-strand break repair
H	0000209	biological_process	protein polyubiquitination
H	0000729	biological_process	DNA double-strand break processing
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005737	cellular_component	cytoplasm
H	0006282	biological_process	regulation of DNA repair
H	0006301	biological_process	postreplication repair
H	0016567	biological_process	protein ubiquitination
H	0031372	cellular_component	UBC13-MMS2 complex
H	0042275	biological_process	error-free postreplication DNA repair
H	0070062	cellular_component	extracellular exosome
H	0070534	biological_process	protein K63-linked ubiquitination
H	1902523	biological_process	positive regulation of protein K63-linked ubiquitination
H	2000781	biological_process	positive regulation of double-strand break repair

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
I	LYS27-ASP52

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	SITE: Interacts with activating enzyme

Chain	Residue	Details
I	ARG54
I	ARG72
C	ARG54
C	ARG72
F	ARG54
F	ARG72

site_id	SWS_FT_FI2
Number of Residues	3
Details	SITE: Essential for function

Chain	Residue	Details
I	HIS68
C	HIS68
F	HIS68

site_id	SWS_FT_FI3
Number of Residues	3
Details	MOD_RES: Phosphoserine; by PINK1 => ECO:0000269\|PubMed:24660806, ECO:0000269\|PubMed:24751536, ECO:0000269\|PubMed:24784582, ECO:0000269\|PubMed:25527291

Chain	Residue	Details
I	SER65
C	SER65
F	SER65
A	ALA92

site_id	SWS_FT_FI4
Number of Residues	3
Details	MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269\|PubMed:32330457

Chain	Residue	Details
I	THR66
C	THR66
F	THR66

site_id	SWS_FT_FI5
Number of Residues	3
Details	MOD_RES: ADP-ribosylglycine => ECO:0000269\|PubMed:28525742

Chain	Residue	Details
I	GLY76
C	GLY76
F	GLY76

site_id	SWS_FT_FI6
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16443603

Chain	Residue	Details
I	LYS6
C	LYS6
F	LYS6

site_id	SWS_FT_FI7
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Chain	Residue	Details
I	GLY76
C	GLY76
F	GLY76

site_id	SWS_FT_FI8
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16443603, ECO:0000269\|PubMed:16543144

Chain	Residue	Details
I	LYS11
I	LYS48
C	LYS11
C	LYS48
F	LYS11
F	LYS48

site_id	SWS_FT_FI9
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305\|PubMed:15466860

Chain	Residue	Details
I	LYS27
C	LYS27
F	LYS27

site_id	SWS_FT_FI10
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16543144, ECO:0000269\|PubMed:25752573, ECO:0000269\|PubMed:25752577, ECO:0000269\|PubMed:34239127

Chain	Residue	Details
I	LYS29
C	LYS29
F	LYS29

site_id	SWS_FT_FI11
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:25752577

Chain	Residue	Details
I	LYS33
C	LYS33
F	LYS33

site_id	SWS_FT_FI12
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16543144, ECO:0000269\|PubMed:18719106

Chain	Residue	Details
I	LYS63
C	LYS63
F	LYS63

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)