7BBD
Crystal structure of monoubiquitinated TRIM21 RING (Ub-RING) In complex with ubiquitin charged Ube2N (Ube2N~Ub) and Ube2V2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | B | Polyubiquitin-B,E3 ubiquitin-protein ligase TRIM21 | polymer | 164 | 18379.2 | 1 | UniProt (J3QS39) UniProt (P19474) Pfam (PF00240) Pfam (PF00097) UniProt (by SIFTS) (P0CG47) In PDB | Homo sapiens (Human) | 52 kDa Ro protein,52 kDa ribonucleoprotein autoantigen Ro/SS-A,RING finger protein 81,RING-type E3 ubiquitin transferase TRIM21,Ro(SS-A),Sjoegren syndrome type A antigen,SS-A,Tripartite motif-containing protein 21 |
| 2 | D | Polyubiquitin-C | polymer | 76 | 8576.8 | 1 | UniProt (P0CG48) Pfam (PF00240) In PDB | Homo sapiens (Human) | |
| 3 | A | Ubiquitin-conjugating enzyme E2 variant 2 | polymer | 150 | 16929.3 | 1 | UniProt (Q15819) Pfam (PF00179) In PDB | Homo sapiens (Human) | DDVit 1,Enterocyte differentiation-associated factor 1,EDAF-1,Enterocyte differentiation-promoting factor 1,EDPF-1,MMS2 homolog,Vitamin D3-inducible protein |
| 4 | C | Ubiquitin-conjugating enzyme E2 N | polymer | 153 | 17182.8 | 1 | UniProt (P61088) Pfam (PF00179) In PDB | Homo sapiens (Human) | Bendless-like ubiquitin-conjugating enzyme,E2 ubiquitin-conjugating enzyme N,Ubc13,UbcH13,Ubiquitin carrier protein N,Ubiquitin-protein ligase N |
| 5 | B | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 6 | water | water | 18.0 | 285 | Chemie (HOH) |
Sequence modifications
B: 1 - 74 (UniProt: J3QS39)
B: 77 - 161 (UniProt: P19474)
A: 1 - 145 (UniProt: Q15819)
C: 1 - 152 (UniProt: P61088)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 75 | - | linker |
| Ala 76 | - | linker |
A: 1 - 145 (UniProt: Q15819)
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Glu -1 | - | expression tag |
| Phe 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Lys 87 | Cys 87 | engineered mutation |
| Ala 92 | Lys 92 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 61068.1 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 130.8 | |
| All* | Total formula weight | 61198.9 |
