7BBD

Crystal structure of monoubiquitinated TRIM21 RING (Ub-RING) In complex with ubiquitin charged Ube2N (Ube2N~Ub) and Ube2V2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	99.150, 108.360, 75.140
Unit cell angles	90.00, 104.99, 90.00

Refinement procedure

Resolution	19.990 - 2.200
R-factor	0.2236
Rwork	0.222
R-free	0.25240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s53 1ubq 1j74
RMSD bond length	0.002
RMSD bond angle	0.525
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.990	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.046	0.946
Rmeas	0.049
Rpim	0.018
Number of reflections	38727	3856
<I/σ(I)>	20.18	2.23
Completeness [%]	99.4	99.15
Redundancy	7.1	7.2
CC(1/2)	1.000	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290.15	MOPSO, Bis-Tris, PEG 4K, 1,2,6-hexanetriol, Li, Na, K