7AY1
Cryo-EM structure of USP1-UAF1 bound to mono-ubiquitinated FANCD2, and FANCI
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Fanconi anemia group I protein | polymer | 1335 | 150459.1 | 1 | UniProt (Q9NVI1) Pfam (PF14674) Pfam (PF14675) Pfam (PF14679) Pfam (PF14676) Pfam (PF14680) Pfam (PF14677) Pfam (PF14678) | Homo sapiens (human) | Protein FACI |
| 2 | B (B) | Fanconi anemia group D2 protein | polymer | 1455 | 164623.8 | 1 | UniProt (Q9BXW9) Pfam (PF14631) | Homo sapiens (human) | Protein FACD2 |
| 3 | C (C) | Ubiquitin-60S ribosomal protein L40 | polymer | 80 | 8875.1 | 1 | UniProt (P62987) Pfam (PF00240) | Homo sapiens (human) | CEP52,Ubiquitin A-52 residue ribosomal protein fusion product 1 |
| 4 | D (D) | Ubiquitin carboxyl-terminal hydrolase 1 | polymer | 786 | 88390.3 | 1 | UniProt (O94782) Pfam (PF00443) | Homo sapiens (human) | Deubiquitinating enzyme 1,hUBP,Ubiquitin thioesterase 1,Ubiquitin-specific-processing protease 1 |
| 5 | E (E) | WD repeat-containing protein 48 | polymer | 694 | 78300.7 | 1 | UniProt (Q8TAF3) Pfam (PF00400) Pfam (PF11816) | Homo sapiens (human) | USP1-associated factor 1,WD repeat endosomal protein,p80 |
| 6 | F, G (S, T) | DNA (61-MER) | polymer | 29 | 5177.8 | 2 | synthetic construct | ||
| 7 | H (D) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
A: 1 - 1328 (UniProt: Q9NVI1)
B: 1 - 1451 (UniProt: Q9BXW9)
C: 1 - 76 (UniProt: P62987)
D: 1 - 785 (UniProt: O94782)
E: 1 - 677 (UniProt: Q8TAF3)
| PDB | External Database | Details |
|---|---|---|
| Met -6 | - | initiating methionine |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Ser 90 | Cys 90 | engineered mutation |
| Ala 670 | Gly 670 | engineered mutation |
| Ala 671 | Gly 671 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -16 | - | initiating methionine |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Leu -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Val -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Phe -5 | - | expression tag |
| Gln -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 501004.6 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 65.4 | |
| All* | Total formula weight | 501070.1 |
