7AAK

Human porphobilinogen deaminase R173W mutant crystallized in the ES2 intermediate state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Porphobilinogen deaminase	polymer	363	39556.2	2	UniProt (P08397) Pfam (PF01379) Pfam (PF03900)	Homo sapiens (Human)	PBG-D,Hydroxymethylbilane synthase,HMBS,Pre-uroporphyrinogen synthase
2	C, F (A, B)	3-[4-(2-hydroxy-2-oxoethyl)-5-[[4-(2-hydroxy-2-oxoethyl)-5-[[4-(2-hydroxy-2-oxoethyl)-5-[[4-(2-hydroxy-2-oxoethyl)-3-(3-hydroxy-3-oxopropyl)-5-methyl-1~{H}-pyrrol-2-yl]methyl]-3-(3-hydroxy-3-oxopropyl)-1~{H}-pyrrol-2-yl]methyl]-3-(3-hydroxy-3-oxopropyl)-1~{H}-pyrrol-2-yl]methyl]-1~{H}-pyrrol-3-yl]propanoic acid	non-polymer		838.8	2	Chemie (7J8)
3	D, E, G, H (A, B)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4	I, J (A, B)	water	water		18.0	505	Chemie (HOH)

Sequence modifications

A, B: 1 - 361 (UniProt: P08397)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Trp 173	Arg 173	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	79112.3
Non-Polymers*	Number of molecules	6
	Total formula weight	2046.0
All*	Total formula weight	81158.3

*Water molecules are not included.