7A08
CryoEM Structure of cGAS Nucleosome complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (a) | Cyclic GMP-AMP synthase | polymer | 370 | 43401.2 | 1 | UniProt (Q8C6L5) Pfam (PF03281) Pfam (PF20266) | Mus musculus (Mouse) | m-cGAS,2'3'-cGAMP synthase,Mab-21 domain-containing protein 1 |
| 2 | B (I) | Nucleosomal DNA strand 1 | polymer | 147 | 45145.8 | 1 | synthetic construct | ||
| 3 | C (J) | Nucleosomal DNA strand 2 | polymer | 147 | 45604.0 | 1 | synthetic construct | ||
| 4 | D, H (b, f) | Histone H2A type 1-C | polymer | 129 | 14004.3 | 2 | UniProt (Q93077) Pfam (PF00125) Pfam (PF16211) | Homo sapiens (Human) | H2A-clustered histone 6,Histone H2A/l |
| 5 | E, I (c, g) | Histone H2B type 1-C/E/F/G/I | polymer | 125 | 13806.0 | 2 | UniProt (P62807) Pfam (PF00125) | Homo sapiens (Human) | Histone H2B.1 A,Histone H2B.a,H2B/a,Histone H2B.g,H2B/g,Histone H2B.h,H2B/h,Histone H2B.k,H2B/k,Histone H2B.l,H2B/l |
| 6 | F, J (d, h) | Histone H3.3 | polymer | 135 | 15229.8 | 2 | UniProt (P84243) Pfam (PF00125) | Homo sapiens (Human) | |
| 7 | G, K (e, i) | Histone H4 | polymer | 102 | 11263.2 | 2 | UniProt (P62805) Pfam (PF15511) | Homo sapiens (Human) | |
| 8 | L (a) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
a: 139 - 507 (UniProt: Q8C6L5)
| PDB | External Database | Details |
|---|---|---|
| Gly 138 | - | expression tag |
| Met 140 | Pro 140 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 242757.7 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 65.4 | |
| All* | Total formula weight | 242823.1 |
