Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7A08

CryoEM Structure of cGAS Nucleosome complex

Functional Information from GO Data

Chain	GOid	namespace	contents
b	0000786	cellular_component	nucleosome
b	0003674	molecular_function	molecular_function
b	0003677	molecular_function	DNA binding
b	0005515	molecular_function	protein binding
b	0005634	cellular_component	nucleus
b	0005654	cellular_component	nucleoplasm
b	0005694	cellular_component	chromosome
b	0008285	biological_process	negative regulation of cell population proliferation
b	0030527	molecular_function	structural constituent of chromatin
b	0031492	molecular_function	nucleosomal DNA binding
b	0031507	biological_process	heterochromatin formation
b	0043229	cellular_component	intracellular organelle
b	0046982	molecular_function	protein heterodimerization activity
b	0070062	cellular_component	extracellular exosome
c	0000786	cellular_component	nucleosome
c	0002227	biological_process	innate immune response in mucosa
c	0003677	molecular_function	DNA binding
c	0005515	molecular_function	protein binding
c	0005615	cellular_component	extracellular space
c	0005634	cellular_component	nucleus
c	0005654	cellular_component	nucleoplasm
c	0005694	cellular_component	chromosome
c	0005829	cellular_component	cytosol
c	0006334	biological_process	nucleosome assembly
c	0019731	biological_process	antibacterial humoral response
c	0030527	molecular_function	structural constituent of chromatin
c	0042742	biological_process	defense response to bacterium
c	0042802	molecular_function	identical protein binding
c	0043229	cellular_component	intracellular organelle
c	0046982	molecular_function	protein heterodimerization activity
c	0050830	biological_process	defense response to Gram-positive bacterium
c	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
c	0070062	cellular_component	extracellular exosome
d	0000775	cellular_component	chromosome, centromeric region
d	0000781	cellular_component	chromosome, telomeric region
d	0000785	cellular_component	chromatin
d	0000786	cellular_component	nucleosome
d	0000939	cellular_component	inner kinetochore
d	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
d	0000979	molecular_function	RNA polymerase II core promoter sequence-specific DNA binding
d	0001556	biological_process	oocyte maturation
d	0001649	biological_process	osteoblast differentiation
d	0001740	cellular_component	Barr body
d	0003677	molecular_function	DNA binding
d	0005515	molecular_function	protein binding
d	0005576	cellular_component	extracellular region
d	0005634	cellular_component	nucleus
d	0005654	cellular_component	nucleoplasm
d	0005694	cellular_component	chromosome
d	0006334	biological_process	nucleosome assembly
d	0006997	biological_process	nucleus organization
d	0007283	biological_process	spermatogenesis
d	0007286	biological_process	spermatid development
d	0007338	biological_process	single fertilization
d	0007566	biological_process	embryo implantation
d	0008283	biological_process	cell population proliferation
d	0008584	biological_process	male gonad development
d	0030307	biological_process	positive regulation of cell growth
d	0030527	molecular_function	structural constituent of chromatin
d	0031492	molecular_function	nucleosomal DNA binding
d	0031508	biological_process	pericentric heterochromatin formation
d	0031509	biological_process	subtelomeric heterochromatin formation
d	0032200	biological_process	telomere organization
d	0032991	cellular_component	protein-containing complex
d	0035264	biological_process	multicellular organism growth
d	0042692	biological_process	muscle cell differentiation
d	0043229	cellular_component	intracellular organelle
d	0046982	molecular_function	protein heterodimerization activity
d	0048477	biological_process	oogenesis
d	0070062	cellular_component	extracellular exosome
d	0090230	biological_process	regulation of centromere complex assembly
d	1902340	biological_process	negative regulation of chromosome condensation
e	0000781	cellular_component	chromosome, telomeric region
e	0000786	cellular_component	nucleosome
e	0003677	molecular_function	DNA binding
e	0003723	molecular_function	RNA binding
e	0005515	molecular_function	protein binding
e	0005576	cellular_component	extracellular region
e	0005634	cellular_component	nucleus
e	0005654	cellular_component	nucleoplasm
e	0005694	cellular_component	chromosome
e	0006325	biological_process	chromatin organization
e	0006334	biological_process	nucleosome assembly
e	0016020	cellular_component	membrane
e	0030527	molecular_function	structural constituent of chromatin
e	0032200	biological_process	telomere organization
e	0032991	cellular_component	protein-containing complex
e	0043505	cellular_component	CENP-A containing nucleosome
e	0045653	biological_process	negative regulation of megakaryocyte differentiation
e	0046982	molecular_function	protein heterodimerization activity
e	0061644	biological_process	protein localization to CENP-A containing chromatin
e	0070062	cellular_component	extracellular exosome
f	0000786	cellular_component	nucleosome
f	0003674	molecular_function	molecular_function
f	0003677	molecular_function	DNA binding
f	0005515	molecular_function	protein binding
f	0005634	cellular_component	nucleus
f	0005654	cellular_component	nucleoplasm
f	0005694	cellular_component	chromosome
f	0008285	biological_process	negative regulation of cell population proliferation
f	0030527	molecular_function	structural constituent of chromatin
f	0031492	molecular_function	nucleosomal DNA binding
f	0031507	biological_process	heterochromatin formation
f	0043229	cellular_component	intracellular organelle
f	0046982	molecular_function	protein heterodimerization activity
f	0070062	cellular_component	extracellular exosome
g	0000786	cellular_component	nucleosome
g	0002227	biological_process	innate immune response in mucosa
g	0003677	molecular_function	DNA binding
g	0005515	molecular_function	protein binding
g	0005615	cellular_component	extracellular space
g	0005634	cellular_component	nucleus
g	0005654	cellular_component	nucleoplasm
g	0005694	cellular_component	chromosome
g	0005829	cellular_component	cytosol
g	0006334	biological_process	nucleosome assembly
g	0019731	biological_process	antibacterial humoral response
g	0030527	molecular_function	structural constituent of chromatin
g	0042742	biological_process	defense response to bacterium
g	0042802	molecular_function	identical protein binding
g	0043229	cellular_component	intracellular organelle
g	0046982	molecular_function	protein heterodimerization activity
g	0050830	biological_process	defense response to Gram-positive bacterium
g	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
g	0070062	cellular_component	extracellular exosome
h	0000775	cellular_component	chromosome, centromeric region
h	0000781	cellular_component	chromosome, telomeric region
h	0000785	cellular_component	chromatin
h	0000786	cellular_component	nucleosome
h	0000939	cellular_component	inner kinetochore
h	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
h	0000979	molecular_function	RNA polymerase II core promoter sequence-specific DNA binding
h	0001556	biological_process	oocyte maturation
h	0001649	biological_process	osteoblast differentiation
h	0001740	cellular_component	Barr body
h	0003677	molecular_function	DNA binding
h	0005515	molecular_function	protein binding
h	0005576	cellular_component	extracellular region
h	0005634	cellular_component	nucleus
h	0005654	cellular_component	nucleoplasm
h	0005694	cellular_component	chromosome
h	0006334	biological_process	nucleosome assembly
h	0006997	biological_process	nucleus organization
h	0007283	biological_process	spermatogenesis
h	0007286	biological_process	spermatid development
h	0007338	biological_process	single fertilization
h	0007566	biological_process	embryo implantation
h	0008283	biological_process	cell population proliferation
h	0008584	biological_process	male gonad development
h	0030307	biological_process	positive regulation of cell growth
h	0030527	molecular_function	structural constituent of chromatin
h	0031492	molecular_function	nucleosomal DNA binding
h	0031508	biological_process	pericentric heterochromatin formation
h	0031509	biological_process	subtelomeric heterochromatin formation
h	0032200	biological_process	telomere organization
h	0032991	cellular_component	protein-containing complex
h	0035264	biological_process	multicellular organism growth
h	0042692	biological_process	muscle cell differentiation
h	0043229	cellular_component	intracellular organelle
h	0046982	molecular_function	protein heterodimerization activity
h	0048477	biological_process	oogenesis
h	0070062	cellular_component	extracellular exosome
h	0090230	biological_process	regulation of centromere complex assembly
h	1902340	biological_process	negative regulation of chromosome condensation
i	0000781	cellular_component	chromosome, telomeric region
i	0000786	cellular_component	nucleosome
i	0003677	molecular_function	DNA binding
i	0003723	molecular_function	RNA binding
i	0005515	molecular_function	protein binding
i	0005576	cellular_component	extracellular region
i	0005634	cellular_component	nucleus
i	0005654	cellular_component	nucleoplasm
i	0005694	cellular_component	chromosome
i	0006325	biological_process	chromatin organization
i	0006334	biological_process	nucleosome assembly
i	0016020	cellular_component	membrane
i	0030527	molecular_function	structural constituent of chromatin
i	0032200	biological_process	telomere organization
i	0032991	cellular_component	protein-containing complex
i	0043505	cellular_component	CENP-A containing nucleosome
i	0045653	biological_process	negative regulation of megakaryocyte differentiation
i	0046982	molecular_function	protein heterodimerization activity
i	0061644	biological_process	protein localization to CENP-A containing chromatin
i	0070062	cellular_component	extracellular exosome

Functional Information from PROSITE/UniProt

site_id	PS00046
Number of Residues	7
Details	HISTONE_H2A Histone H2A signature. AGLqFPV

Chain	Residue	Details
b	ALA21-VAL27

site_id	PS00047
Number of Residues	5
Details	HISTONE_H4 Histone H4 signature. GAKRH

Chain	Residue	Details
e	GLY14-HIS18

site_id	PS00322
Number of Residues	7
Details	HISTONE_H3_1 Histone H3 signature 1. KAPRKQL

Chain	Residue	Details
d	LYS14-LEU20

site_id	PS00357
Number of Residues	23
Details	HISTONE_H2B Histone H2B signature. REIQTavRlLLpGELaKHAVSEG

Chain	Residue	Details
c	ARG92-GLY114

site_id	PS00959
Number of Residues	9
Details	HISTONE_H3_2 Histone H3 signature 2. PFqRLVREI

Chain	Residue	Details
d	PRO66-ILE74

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	DNA_BIND:

Chain	Residue	Details
e	LYS16-LYS20
i	LYS16-LYS20
a	GLU211
a	ASP213
a	ASP307
a	GLU371
a	LYS402
a	SER420

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000269\|PubMed:17967882

Chain	Residue	Details
e	SER1
i	SER1
a	ARG364

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Symmetric dimethylarginine; by PRMT5 and PRMT7; alternate => ECO:0000250\|UniProtKB:P62806

Chain	Residue	Details
e	ARG3
g	LYS11
g	LYS15
g	LYS16
g	LYS20
g	LYS23
g	LYS43
g	LYS85
i	ARG3
c	LYS15
c	LYS16
c	LYS20
c	LYS23
c	LYS43
c	LYS85
g	LYS5

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: N6-lactoyllysine; alternate => ECO:0000269\|PubMed:31645732

Chain	Residue	Details
e	LYS5
i	LYS5
f	LYS9
f	LYS95

site_id	SWS_FT_FI5
Number of Residues	6
Details	MOD_RES: N6-propionyllysine; alternate => ECO:0000269\|PubMed:17267393

Chain	Residue	Details
e	LYS8
e	LYS16
e	LYS44
i	LYS8
i	LYS16
i	LYS44

site_id	SWS_FT_FI6
Number of Residues	8
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000269\|PubMed:22389435

Chain	Residue	Details
e	LYS12
e	LYS31
e	LYS77
e	LYS91
i	LYS12
i	LYS31
i	LYS77
i	LYS91

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: N6-methyllysine; alternate => ECO:0000269\|PubMed:12086618, ECO:0000269\|PubMed:15964846, ECO:0000269\|PubMed:17967882, ECO:0000269\|PubMed:27338793

Chain	Residue	Details
e	LYS20
i	LYS20
f	LYS74
f	LYS75

site_id	SWS_FT_FI8
Number of Residues	2
Details	MOD_RES: Phosphoserine; by PAK2 => ECO:0000269\|PubMed:21724829, ECO:0007744\|PubMed:17081983, ECO:0007744\|PubMed:18669648, ECO:0007744\|PubMed:19690332, ECO:0007744\|PubMed:20068231, ECO:0007744\|PubMed:21406692, ECO:0007744\|PubMed:23186163, ECO:0007744\|PubMed:24275569

Chain	Residue	Details
e	SER47
i	SER47
c	LYS120
g	LYS34
g	LYS116
g	LYS120

site_id	SWS_FT_FI9
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0007744\|PubMed:15592455, ECO:0007744\|PubMed:20068231

Chain	Residue	Details
e	TYR51
i	TYR51
b	LYS125
f	LYS118
f	LYS119
f	LYS125

site_id	SWS_FT_FI10
Number of Residues	2
Details	MOD_RES: N6-glutaryllysine; alternate => ECO:0000269\|PubMed:31542297

Chain	Residue	Details
e	LYS59
i	LYS59

site_id	SWS_FT_FI11
Number of Residues	2
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250\|UniProtKB:P62806

Chain	Residue	Details
e	LYS79
i	LYS79
h	LYS14
h	LYS56

site_id	SWS_FT_FI12
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0000250\|UniProtKB:P62806

Chain	Residue	Details
e	THR80
i	THR80
f	LYS119

site_id	SWS_FT_FI13
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0007744\|PubMed:24275569

Chain	Residue	Details
e	TYR88
i	TYR88
h	LYS18
h	LYS64

site_id	SWS_FT_FI14
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744\|PubMed:25218447

Chain	Residue	Details
e	LYS12
i	LYS12
g	ARG86
g	ARG92

site_id	SWS_FT_FI15
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000269\|PubMed:19818714

Chain	Residue	Details
d	ARG26
e	LYS91
i	LYS91

site_id	SWS_FT_FI16
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744\|PubMed:28112733

Chain	Residue	Details
e	LYS20
e	LYS59
e	LYS79
i	LYS20
i	LYS59
i	LYS79

site_id	SWS_FT_FI17
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in UFM1); alternate => ECO:0000269\|PubMed:30886146

Chain	Residue	Details
e	LYS31
i	LYS31
a	CYS459

site_id	SWS_FT_FI18
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000269\|PubMed:15851689, ECO:0000269\|PubMed:16185088

Chain	Residue	Details
d	SER31
h	SER31
g	LYS120

site_id	SWS_FT_FI19
Number of Residues	2
Details	MOD_RES: N6-methyllysine => ECO:0000250\|UniProtKB:P68431

Chain	Residue	Details
d	LYS37
h	LYS37

site_id	SWS_FT_FI20
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000269\|PubMed:19783980

Chain	Residue	Details
d	TYR41
h	TYR41

site_id	SWS_FT_FI21
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000269\|PubMed:20850016

Chain	Residue	Details
d	SER57
h	SER57

site_id	SWS_FT_FI22
Number of Residues	2
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000269\|PubMed:22389435, ECO:0000269\|PubMed:29211711

Chain	Residue	Details
d	LYS79
h	LYS79

site_id	SWS_FT_FI23
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0000269\|PubMed:20850016

Chain	Residue	Details
d	THR80
h	THR80

site_id	SWS_FT_FI24
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:24275569

Chain	Residue	Details
d	SER86
h	SER86

site_id	SWS_FT_FI25
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0000250\|UniProtKB:Q71DI3

Chain	Residue	Details
d	THR107
h	THR107

site_id	SWS_FT_FI26
Number of Residues	2
Details	MOD_RES: N6-glutaryllysine; alternate => ECO:0000269\|PubMed:31542297

Chain	Residue	Details
d	LYS115
h	LYS115

site_id	SWS_FT_FI27
Number of Residues	2
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000269\|PubMed:22389435, ECO:0000269\|PubMed:27436229

Chain	Residue	Details
d	LYS122
h	LYS122

site_id	SWS_FT_FI28
Number of Residues	2
Details	LIPID: N6-decanoyllysine => ECO:0000269\|PubMed:35939806

Chain	Residue	Details
d	LYS18
h	LYS18

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)