6ZYU

Structure of the GluA2 ligand-binding domain (L483Y-N754S) in complex with glutamate and BPAM549

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Glutamate receptor 2,Glutamate receptor 2	polymer	264	29301.7	3	UniProt (P19491) Pfam (PF10613) Pfam (PF00060) In PDB	Rattus norvegicus (Rat)	GluR-2,AMPA-selective glutamate receptor 2,GluR-B,GluR-K2,Glutamate receptor ionotropic,AMPA 2,GluA2,GluR-2,AMPA-selective glutamate receptor 2,GluR-B,GluR-K2,Glutamate receptor ionotropic,AMPA 2,GluA2
2	C, A, B	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
3	A	CACODYLATE ION	non-polymer		137.0	1	Chemie (CAC)
4	C, A, B	ACETATE ION	non-polymer		59.0	5	Chemie (ACT)
5	C, A, B	GLUTAMIC ACID	non-polymer		147.1	3	Chemie (GLU)
6	C, A, B	(4~{S})-4-cyclopropyl-7-fluoranyl-3,4-dihydro-2~{H}-thiochromene 1,1-dioxide	non-polymer		240.3	3	Chemie (QSZ)
7	C, A, B	(4~{R})-4-cyclopropyl-7-fluoranyl-3,4-dihydro-2~{H}-thiochromene 1,1-dioxide	non-polymer		240.3	3	Chemie (QSW)
8	C, A	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
9	B, C, A	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
10		water	water		18.0	532	Chemie (HOH)

Sequence modifications

A, B, C: 3 - 117 (UniProt: P19491)

PDB	External Database	Details
Gly 1	-	expression tag
Ala 2	-	expression tag
Tyr 94	Leu 504	engineered mutation

A, B, C: 120 - 264 (UniProt: P19491)

PDB	External Database	Details
Gly 118	-	linker
Thr 119	-	linker
Ser 242	Asn 775	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	87905.2
Non-Polymers*	Number of molecules	34
	Total formula weight	3568.5
All*	Total formula weight	91473.6

*Water molecules are not included.