6ZYU

Structure of the GluA2 ligand-binding domain (L483Y-N754S) in complex with glutamate and BPAM549

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 2
Unit cell lengths	114.324, 163.084, 47.449
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.094 - 1.900
R-factor	0.1637
Rwork	0.162
R-free	0.18680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tkd
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (1.14)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.094	49.094	2.000
High resolution limit [Å]	1.900	6.010	1.900
Rmerge		0.045	0.603
Rmeas	0.095	0.049	0.653
Rpim	0.037	0.019	0.248
Total number of observations		15083	69915
Number of reflections	71004	2485	10193
<I/σ(I)>	12.4	26.8	3.3
Completeness [%]	100.0	99.9	100
Redundancy	6.7	6.1	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	299	Zinc acetate, polyethylene glycol 4000, cacodylate