6ZYU
Structure of the GluA2 ligand-binding domain (L483Y-N754S) in complex with glutamate and BPAM549
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 114.324, 163.084, 47.449 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.094 - 1.900 |
| R-factor | 0.1637 |
| Rwork | 0.162 |
| R-free | 0.18680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tkd |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (1.14) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.094 | 49.094 | 2.000 |
| High resolution limit [Å] | 1.900 | 6.010 | 1.900 |
| Rmerge | 0.045 | 0.603 | |
| Rmeas | 0.095 | 0.049 | 0.653 |
| Rpim | 0.037 | 0.019 | 0.248 |
| Total number of observations | 15083 | 69915 | |
| Number of reflections | 71004 | 2485 | 10193 |
| <I/σ(I)> | 12.4 | 26.8 | 3.3 |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 6.7 | 6.1 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 299 | Zinc acetate, polyethylene glycol 4000, cacodylate |
