6ZX9
Crystal structure of SIV Vpr,fused to T4 lysozyme, isolated from moustached monkey, bound to human DDB1 and human DCAF1 (amino acid residues 1046-1396)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA damage-binding protein 1 | polymer | 1142 | 127241.6 | 1 | UniProt (Q16531) Pfam (PF10433) Pfam (PF03178) | Homo sapiens (Human) | DDB p127 subunit,DNA damage-binding protein a,DDBa,Damage-specific DNA-binding protein 1,HBV X-associated protein 1,XAP-1,UV-damaged DNA-binding factor,UV-damaged DNA-binding protein 1,UV-DDB 1,XPE-binding factor,XPE-BF,Xeroderma pigmentosum group E-complementing protein,XPCe |
| 2 | B (B) | DDB1- and CUL4-associated factor 1 | polymer | 360 | 40876.1 | 1 | UniProt (Q9Y4B6) | Homo sapiens (Human) | HIV-1 Vpr-binding protein,VprBP,Serine/threonine-protein kinase VPRBP,Vpr-interacting protein |
| 3 | C (C) | Vpr protein fused to T4 lysozyme | polymer | 258 | 29703.8 | 1 | UniProt (P00720) UniProt (A4UDG5) Pfam (PF00959) Pfam (PF00522) | Enterobacteria phage T4 (Bacteriophage T4) | Lysis protein,Lysozyme,Muramidase |
| 4 | D, E, F, G, H... (A, B, C) | GLYCEROL | non-polymer | 92.1 | 8 | Chemie (GOL) | |||
| 5 | K (C) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 6 | M, N, O (A, B, C) | water | water | 18.0 | 253 | Chemie (HOH) |
Sequence modifications
A: 1 - 1140 (UniProt: Q16531)
B: 1046 - 1396 (UniProt: Q9Y4B6)
C: -165 - -3 (UniProt: P00720)
C: 1 - 92 (UniProt: A4UDG5)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 1045 | - | initiating methionine |
| Glu 1397 | - | expression tag |
| Leu 1398 | - | expression tag |
| Ala 1399 | - | expression tag |
| Leu 1400 | - | expression tag |
| Val 1401 | - | expression tag |
| Pro 1402 | - | expression tag |
| Arg 1403 | - | expression tag |
| Gly 1404 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| His -156 | Glu 11 | conflict |
| Gly -155 | Arg 12 | conflict |
| Thr -113 | Cys 54 | conflict |
| Ala -70 | Cys 97 | conflict |
| Arg -30 | Ile 137 | conflict |
| Ala -2 | - | linker |
| Ala -1 | - | linker |
| Ala 0 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 197821.5 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 802.2 | |
| All* | Total formula weight | 198623.6 |
