6ZRN

Crystal structure of the RLIP76 Ral binding domain mutant (E427S/L429M/Q433L/K440R) in complex with RalB-GMPPNP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Ras-related protein Ral-B	polymer	185	20998.7	2	UniProt (P11234) Pfam (PF00071) In PDB	Homo sapiens (Human)
2	C, D	RalA-binding protein 1	polymer	59	6856.9	2	UniProt (Q15311) Pfam (PF20924) In PDB	Homo sapiens (Human)	RalBP1,76 kDa Ral-interacting protein,Dinitrophenyl S-glutathione ATPase,DNP-SG ATPase,Ral-interacting protein 1
3	A, B	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	2	Chemie (GNP)
4	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	A, C	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6		water	water		18.0	244	Chemie (HOH)

Sequence modifications

A, B: 1 - 185 (UniProt: P11234)

PDB	External Database	Details
Leu 72	Gln 72	engineered mutation

C, D: 393 - 446 (UniProt: Q15311)

PDB	External Database	Details
Gly 388	-	expression tag
Pro 389	-	expression tag
Leu 390	-	expression tag
Gly 391	-	expression tag
Ser 392	-	expression tag
Ser 411	Cys 411	engineered mutation
Ser 427	Glu 427	engineered mutation
Met 429	Leu 429	engineered mutation
Leu 433	Gln 433	engineered mutation
Arg 440	Lys 440	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	55711.3
Non-Polymers*	Number of molecules	6
	Total formula weight	1277.2
All*	Total formula weight	56988.4

*Water molecules are not included.