6ZRN

Crystal structure of the RLIP76 Ral binding domain mutant (E427S/L429M/Q433L/K440R) in complex with RalB-GMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-10-14
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	47.196, 77.517, 65.767
Unit cell angles	90.00, 90.07, 90.00

Refinement procedure

Resolution	50.150 - 1.482
R-factor	0.2101
Rwork	0.209
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2kwi
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.770	65.770	1.560
High resolution limit [Å]	1.480	4.690	1.480
Rmerge	0.255	0.136	6.167
Rmeas	0.264	0.141	6.376
Rpim	0.069	0.037	1.604
Total number of observations	1159648	34338	176059
Number of reflections	78582	2563	11442
<I/σ(I)>	8	20.5	1.3
Completeness [%]	100.0	99.9	100
Redundancy	14.8	13.4	15.4
CC(1/2)	0.996	0.993	0.446

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Bicine 9.0 pH, 30% w/v PEG 6000