6ZRN
Crystal structure of the RLIP76 Ral binding domain mutant (E427S/L429M/Q433L/K440R) in complex with RalB-GMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-10-14 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.196, 77.517, 65.767 |
Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
Resolution | 50.150 - 1.482 |
R-factor | 0.2101 |
Rwork | 0.209 |
R-free | 0.23850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2kwi |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 65.770 | 65.770 | 1.560 |
High resolution limit [Å] | 1.480 | 4.690 | 1.480 |
Rmerge | 0.255 | 0.136 | 6.167 |
Rmeas | 0.264 | 0.141 | 6.376 |
Rpim | 0.069 | 0.037 | 1.604 |
Total number of observations | 1159648 | 34338 | 176059 |
Number of reflections | 78582 | 2563 | 11442 |
<I/σ(I)> | 8 | 20.5 | 1.3 |
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 14.8 | 13.4 | 15.4 |
CC(1/2) | 0.996 | 0.993 | 0.446 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bicine 9.0 pH, 30% w/v PEG 6000 |