6ZEH

Structure of PP1-spectrin alpha II chimera [PP1(7-304) + linker (G/S)x9 + spectrin alpha II (1025-1039)] bound to Phactr1 (516-580)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Serine/threonine-protein phosphatase PP1-alpha catalytic subunit,Spectrin alpha chain, non-erythrocytic 1	polymer	336	37558.6	2	UniProt (P62136) UniProt (Q13813) Pfam (PF16891) Pfam (PF00149)	Homo sapiens (Human)	PP-1A,Alpha-II spectrin,Fodrin alpha chain,Spectrin,non-erythroid alpha subunit
2	B, D (C, D)	Phosphatase and actin regulator	polymer	70	8257.3	2	UniProt (Q4VY12) UniProt (by SIFTS) (Q9C0D0)	Homo sapiens (Human)
3	E, F, H, I (A, B)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
4	G, J (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
5	K (B)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
6	L, M, N, O (A, C, B, D)	water	water		18.0	655	Chemie (HOH)

Sequence modifications

A, B: 7 - 304 (UniProt: P62136)

PDB	External Database	Details
Gly 2	-	expression tag
His 3	-	expression tag
Met 4	-	expression tag
Gly 5	-	expression tag
Ser 6	-	expression tag
Ser 305	-	linker
Gly 306	-	linker
Ser 307	-	linker
Gly 308	-	linker
Ser 309	-	linker
Gly 310	-	linker
Ser 311	-	linker
Gly 312	-	linker
Ser 313	-	linker
Gly 314	-	linker
Ser 315	-	linker
Gly 316	-	linker
Ser 317	-	linker
Gly 318	-	linker
Ser 319	-	linker
Gly 320	-	linker
Ser 321	-	linker
Gly 322	-	linker

A, B: 323 - 337 (UniProt: Q13813)
C, D: 516 - 580 (UniProt: Q4VY12)

PDB	External Database	Details
Gly 511	-	expression tag
Pro 512	-	expression tag
Leu 513	-	expression tag
Gly 514	-	expression tag
Ser 515	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	91631.8
Non-Polymers*	Number of molecules	7
	Total formula weight	505.8
All*	Total formula weight	92137.6

*Water molecules are not included.