6ZEH

Structure of PP1-spectrin alpha II chimera [PP1(7-304) + linker (G/S)x9 + spectrin alpha II (1025-1039)] bound to Phactr1 (516-580)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.968
Spacegroup name	P 1 21 1
Unit cell lengths	48.501, 122.388, 69.343
Unit cell angles	90.00, 92.18, 90.00

Refinement procedure

Resolution	69.290 - 1.300
R-factor	0.1384
Rwork	0.137
R-free	0.16930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mov
RMSD bond length	0.010
RMSD bond angle	1.146
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.290	1.340
High resolution limit [Å]	1.300	1.300
Rmerge	0.138	1.780
Rmeas	0.150	1.940
Rpim	0.058	0.750
Number of reflections	196809	123745
<I/σ(I)>	5.89	1.12
Completeness [%]	99.1
Redundancy	6.5
CC(1/2)	0.994	0.475

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	20% (w/v) polyethylene glycol 3350, 0.2 M NaI and 0.1 M BIS-Tris propane pH 8.5