6Z9U

Crystal structure of a TSEN15-34 heterodimer.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	tRNA-splicing endonuclease subunit Sen34	polymer	104	11760.4	2	UniProt (Q9BSV6) Pfam (PF01974)	Homo sapiens (Human)	Leukocyte receptor cluster member 5,tRNA-intron endonuclease Sen34,HsSen34
2	B, D (B, D)	tRNA-splicing endonuclease subunit Sen15	polymer	167	18748.2	2	UniProt (Q8WW01) Pfam (PF09631)	Homo sapiens (Human)	SEN15 homolog,HsSEN15,tRNA-intron endonuclease Sen15
3	E, F (D)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	G, H, I, J (A, B, C, D)	water	water		18.0	108	Chemie (HOH)

Sequence modifications

A, C: 208 - 310 (UniProt: Q9BSV6)

PDB	External Database	Details
Met 207	-	initiating methionine

B, D: 23 - 170 (UniProt: Q8WW01)

PDB	External Database	Details
Met 4	-	initiating methionine
Gly 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
Gly 16	-	expression tag
Glu 17	-	expression tag
Asn 18	-	expression tag
Leu 19	-	expression tag
Tyr 20	-	expression tag
Phe 21	-	expression tag
Gln 22	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	61017.2
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	61201.4

*Water molecules are not included.