6Z9U

Crystal structure of a TSEN15-34 heterodimer.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97930
Spacegroup name	P 1 21 1
Unit cell lengths	34.848, 69.277, 94.790
Unit cell angles	90.00, 98.31, 90.00

Refinement procedure

Resolution	28.257 - 2.100
R-factor	0.194805337095
Rwork	0.192
R-free	0.25285
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p1z
RMSD bond length	0.009
RMSD bond angle	0.986
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.260	2.175
High resolution limit [Å]	2.100	2.100
Number of reflections	25897	2594
<I/σ(I)>	13.37
Completeness [%]	99.0	99
Redundancy	5.9
CC(1/2)	0.999	0.818

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	imidazole, 2-(N-morpholino)ethanesulfonic acid, PEG 3350, magnesium chloride