6Z10

Crystal structure of a humanized (K18E, K269N) rat succinate receptor SUCNR1 (GPR91) in complex with a nanobody and antagonist

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Succinate receptor 1	polymer	342	39827.7	1	UniProt (Q6IYF9) Pfam (PF00001) In PDB	Rattus norvegicus (Rat)	G-protein coupled receptor 91
2	B	Nanobody6	polymer	142	15527.1	1		Vicugna pacos
3	A	2-[2-[[3-[4-chloranyl-2-fluoranyl-5-[(3~{R})-piperidin-3-yl]oxy-phenyl]-2-fluoranyl-phenyl]carbonylamino]-5-fluoranyl-phenyl]ethanoic acid	non-polymer		518.9	1	Chemie (Q4T)
4	A	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	12	Chemie (OLC)
5	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
6	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7		water	water		18.0	154	Chemie (HOH)

Sequence modifications

A: 2 - 317 (UniProt: Q6IYF9)

PDB	External Database	Details
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag
Glu 18	Lys 18	engineered mutation
Asn 269	Lys 269	engineered mutation
Leu 318	-	expression tag
Glu 319	-	expression tag
Val 320	-	expression tag
Leu 321	-	expression tag
Phe 322	-	expression tag
Gln 323	-	expression tag
Gly 324	-	expression tag
Pro 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag
His 331	-	expression tag
His 332	-	expression tag
His 333	-	expression tag
His 334	-	expression tag
His 335	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55354.8
Non-Polymers*	Number of molecules	15
	Total formula weight	4928.9
All*	Total formula weight	60283.7

*Water molecules are not included.