6YSC

GLYCOSYLATED KNOB-HOLE/DUMMY FC FRAGMENT

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Immunoglobulin gamma-1 heavy chain	polymer	227	25682.1	1	UniProt (P0DOX5)	Homo sapiens (Human)	Immunoglobulin gamma-1 heavy chain NIE
2	B (B)	Immunoglobulin gamma-1 heavy chain	polymer	241	26984.5	1	UniProt (P0DOX5)	Homo sapiens (Human)	Immunoglobulin gamma-1 heavy chain NIE
3	C, D (C, D)	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1422.3	2	In PDB GlyTouCan (G61334IA)
4	E, F (A, B)	water	water		18.0	81	Chemie (HOH)

Sequence modifications

A: 221 - 447 (UniProt: P0DOX5)

PDB	External Database	Details
Cys 354	Ser 356	engineered mutation
Trp 366	Thr 368	engineered mutation

B: 221 - 447 (UniProt: P0DOX5)

PDB	External Database	Details
Lys 357	Glu 359	engineered mutation
Ser 366	Thr 368	engineered mutation
Ala 368	Leu 370	engineered mutation
Val 407	Tyr 409	engineered mutation
Gly 448	-	expression tag
Gly 449	-	expression tag
Gly 450	-	expression tag
Gly 451	-	expression tag
Ser 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
His 455	-	expression tag
His 456	-	expression tag
His 457	-	expression tag
His 458	-	expression tag
His 459	-	expression tag
His 460	-	expression tag
Cys 461	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	52666.6
Branched	Number of molecules	2
	Total formula weight	2844.6
All*	Total formula weight	55511.2

*Water molecules are not included.