6YSC

GLYCOSYLATED KNOB-HOLE/DUMMY FC FRAGMENT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	49.361, 80.357, 141.826
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.010 - 2.050
Rwork	0.230
R-free	0.29286
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hy9
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.010	2.210
High resolution limit [Å]	2.050	2.050
Rpim	0.024	0.423
Number of reflections	24877	1310
<I/σ(I)>	17.4	1.5
Completeness [%]	94.8
Redundancy	6.5
CC(1/2)	1.000	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294	15% (w/v) PEG4000, 0.1M HEPES