6Y3Z

Crystal structure of the Pby1 ATP-grasp enzyme bound to the S. cerevisiae mRNA decapping complex (Dcp1-Dcp2-Edc3)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	m7GpppN-mRNA hydrolase	polymer	277	32946.9	1	UniProt (P53550) Pfam (PF05026) Pfam (PF00293)	Saccharomyces cerevisiae S288C	Protein PSU1,mRNA-decapping enzyme subunit 2
2	B (B)	mRNA-decapping enzyme subunit 1	polymer	236	26705.8	1	UniProt (Q12517) Pfam (PF06058)	Saccharomyces cerevisiae S288C
3	C (C)	Enhancer of mRNA-decapping protein 3	polymer	71	7448.6	1	UniProt (P39998)	Saccharomyces cerevisiae S288C
4	D (P)	Probable tubulin--tyrosine ligase PBY1	polymer	429	49895.7	1	UniProt (P38254) Pfam (PF03133)	Saccharomyces cerevisiae S288C	P-body-associated protein 1
5	E (P)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 1 - 271 (UniProt: P53550)

PDB	External Database	Details
His 272	-	expression tag
His 273	-	expression tag
His 274	-	expression tag
His 275	-	expression tag
His 276	-	expression tag
His 277	-	expression tag

B: 1 - 231 (UniProt: Q12517)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

C: 1 - 66 (UniProt: P39998)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

P: 330 - 753 (UniProt: P38254)

PDB	External Database	Details
Gly 325	-	expression tag
Pro 326	-	expression tag
Leu 327	-	expression tag
Gly 328	-	expression tag
Ser 329	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	116997.0
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	117021.3

*Water molecules are not included.