6Y3Z

Crystal structure of the Pby1 ATP-grasp enzyme bound to the S. cerevisiae mRNA decapping complex (Dcp1-Dcp2-Edc3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97626
Spacegroup name	P 21 21 21
Unit cell lengths	71.210, 90.600, 194.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.500 - 3.490
R-factor	0.25
Rwork	0.246
R-free	0.32100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k6e 6y3p 5lop
RMSD bond length	0.010
RMSD bond angle	1.270
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	3.550
High resolution limit [Å]	3.490	3.490
Rmerge	0.101	1.409
Rpim	0.034	0.479
Number of reflections	16546	828
<I/σ(I)>	9.6	1.3
Completeness [%]	99.5
Redundancy	10.1
CC(1/2)	0.999	0.070

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.05 M calcium acetate; 0.1 M sodium cacodylate pH6; 25% MPD